肾病综合征患者细胞免疫和细胞因子的变化及其意义  

Changes of Cell-mediated Immunity and Cytokines with Nephrotic Syndrome

作　　者：赵学兰[1] 张琳琳[1] 李凯[1] 王金玲[1] 刘晓霞[1] 肖青[1] 

机构地区：[1]潍坊医学院附属医院肾内科,山东潍坊261031

出　　处：《潍坊医学院学报》2008年第1期11-13,共3页Acta Academiae Medicinae Weifang

摘　　要：目的探讨肾病综合征(NS)患者T细胞免疫功能和TNF-α与NS发病机制的关系。方法应用流式细胞术和酶联免疫吸附(ELISA)方法,对32例NS患者在活动期和20例缓解期以及18例正常人外周血T细胞亚群以及血清TNF-α水平进行测定。结果血清TNFα水平在活动期明显高于缓解期和对照组,缓解期高于对照组。NS活动期外周血总CD3+,CD4+,CD4+/CD8+细胞比值较缓解期和对照组显著降低,CD8+细胞增高。NS活动期TNF-α与CD3+,CD4+,CD4+/CD8+呈负相关,与CD8+细胞呈正相关。结论NS患者在活动期存在细胞因子失调,T细胞亚群的比例结构发生变化,细胞免疫功能异常及细胞因子网络失调在NS发病中起一定作用。Objective To evaluate the relationship among cell-mediated immunological function and pathogenesis of nephrotic syndrome（NS）. Methods Peripheral T cell subset was assayed in 32 active phase NS,20 remission phase and 18 control group by flow eytometry and their TNF-α levels in serum were detected by ELISA. Results TNF-α level in active phase NS were significantly higher than those in remission phase anti control group,in remission phase higher than in control group. The total CD3^ ＋ ,CD4 ^＋ and the ratio of CD4^ ＋/ CD8^ ＋ in peripheral blood decreased,while C D8^ ＋ increased. In active phase NS,TNF-α correlated negatively with （CD3^＋ ,CD4 ^＋and CD4^＋ / CD8^＋ , while positively with CD8^＋ ＋ . Conclusion Imbalance of TNF-α and change of proportion of T cell subset couht he detected in patients with active phase NS. It was the disturbance of cell-mediated immunological function and imhalance of cytokines network that play, a role iu the pathogenesis of NS.

关 键 词：肾病综合征 T细胞亚群 肿瘤坏死因子 

分 类 号：R692[医药卫生—泌尿科学]