大黄素对大鼠肝移植后肝细胞凋亡的影响  

Effect of Emodin on Hepatocellular Apoptosis Following Orthotopic Liver Transplantation in Rats

作　　者：徐贤绸[1] 林胜璋[1] 童洪飞[1] 高宝辉[2] 杨潇[1] 游涛[1] 

机构地区：[1]温州医学院附属第二医院外科,温州325003 [2]温州医学院附属第二医院病理科,温州325003

出　　处：《中国中西医结合外科杂志》2008年第2期131-134,共4页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金(30572395)

摘　　要：目的:探讨大黄素对大鼠肝移植后肝细胞凋亡的影响。方法:建立LEW→BN大鼠肝移植动物模型,随机分为A组(排斥反应组)、B组(CsA组)、C组(大黄素组)、D组(CsA+大黄素组)4组,每组6只。术后每天分别腹腔注射生理盐水0.5mL/d、环孢素A(CsA)10.0mg/(kg.d)、大黄素50.0mg/(kg.d)、大黄素50.0mg/(kg.d)+CsA10.0mg/(kg.d)。连续用药8d,停药后处死动物取肝脏组织观察肝细胞凋亡指数(AI)和排斥活动指数(RAI)。结果:A、B、C、D组AI分别是35.83±2.32、15.83±1.33、16.50±2.35、11.50±1.05,RAI分别是7.67±0.98、5.17±0.40、5.83±0.75、3.83±0.75。B、C、D组的AI、RAI较A组均明显降低(P<0.01),而D组较B、C组也均有明显降低(P<0.05),B、C组间差别均无意义(P>0.05)。结论:大黄素能有效减少大鼠移植肝术后肝细胞凋亡,抑制排斥反应。Objective To evaluate the effect of emodin on hepatocellular apoptosis following orthotopic liver transplantation （OLT） in rats. Methods The LEW→BN OLT models were established. A total of 24 rats were divided randomly and equally into 4 groups： group A was treated with normal saline at dose of 0.5 ml· d^-1 intraperitoneally from 1 st day to 8th day after operation; Group B, cyclosporin A（CsA） at dose of 10.0 mg· kg^-1· d^-1 ; Group C, emodin at dose of 50.0 mg· kg^-1· d^-1 ; Group D, CsA at dose of 10.0 mg· kg^-1· d^- 1 and emodin at dose of 50.0 mg· kg^-1· d^- 1. Eight days after operation, hepatocellular apoptosis index （AI） and Rejection active index （RAI） was confirmed after observing the pathologic changes of transplanted liver in recipients. Results Respectively, the AI of group A, B, C, D was 35.83 ± 2.32, 15.83 ± 1.33, 16.50 ± 2.35 and 11.50 ± 1.05 and the RAI of group A,B,C,D was 7.67 ± 0.98,5.17 ± 0. 40,5.83 ±0.75 and 3.83 ± 0.75.The AI and RAI of group B,C,D were significantly lower than those of group A （P 〈 0.01 ）and those of group D were significantly lower than of group B and C too（ P 〈 0.05）. There was no significant distinction between group B and C in RAI and AI（ P 〉 0.05）. Conclusion Emodin has the effect of reducing the hep- tocellular apoptosis following OLT in rats and the effect can be enhanced by CsA.

关 键 词：大黄素 肝移植 移植物排斥 细胞凋亡 

分 类 号：R258.6[医药卫生—中医内科学] R698.2[医药卫生—中医学]