有机溶剂对黑根霉甾体11α-羟基化的影响  

Effects of organic solvents on 11α-hydroxylation of steroid by Rhizopus nigricans

作　　者：周浩力[1] 张妍[1] 卢文玉[1] 闻建平[1] 

机构地区：[1]天津大学化工学院,天津300072

出　　处：《工业微生物》2008年第2期52-55,共4页Industrial Microbiology

摘　　要：考察了有机溶剂对黑根霉甾体11α-羟基化反应中转化底物16α,17α-环氧孕甾酮生成11α,16α,17α-环氧孕甾酮的转化率和细胞色素P450酶浓度的影响。向培养28 h的培养液中添加终浓度0.035 mol/L的丙酮和1.75 g/L的底物,继续转化48 h。与未添加丙酮相比,添加丙酮后的底物转化率和细胞色素P450酶表达量分别提高了6.8%和30%。说明丙酮添加可明显提高黑根霉甾体11α-羟基化能力和细胞色素P450酶的表达。The effects of organic solvent on the conversion of substrate 11α, 16α, 17α progesterone epoxy and the cytochrome P450 content by Rhizopus nigricans were investigated. The conversion rate and the P450 content were increased by 6.8 % and 30 %, respectively, when 0. 035 mol/L final concentration acetone was added into the culture medium at 28 h comparing with the contrast. The results showed that acetone could significantly enhance the 11α-hydroxylation ability and the cytochrome P450 expression by Rhizopus nigricans.

关 键 词：有机溶剂 黑根霉 转化率 细胞色素P450 

分 类 号：TQ460.1[化学工程—制药化工]