红树林放线菌的分离及其抗菌和抗肿瘤细胞活性  

Isolation of Mangrove Actinomycetes and Their Antagonistic Activities

作　　者：肖静[1] 许静[2] 谢庶洁[2] 张熙颖[2] 喻子牛[1] 徐俊[2] 

机构地区：[1]华中农业大学生命科学技术学院农业微生物学国家重点实验室,武汉430070 [2]国家海洋局第三海洋研究所海洋生物遗传资源重点实验室,福建厦门361005

出　　处：《应用与环境生物学报》2008年第2期244-248,共5页Chinese Journal of Applied and Environmental Biology

基　　金：国家高技术研究发展计划专项经费资助(863项目,No.2006AA09Z437);福建省青年科技人才创新项目(2005J063)~~

摘　　要：从福建漳州红树林保护区采集土壤样品中分离到163株放线菌,用5种指示菌(Bacillus subtilis,Staphylococ-cus aureus,Escherichia coli,Candida albicans and Rhizoctonia solani)测定了它们的抗菌活性,结果表明,其中42·3%的放线菌发酵液具有单一或多种抗菌活性.同时测定了它们的发酵液对肝癌细胞BEL7402、肺癌细胞A549和白血病细胞HL60三种肿瘤细胞的毒性,其中37·4%的放线菌发酵液具有单一或多种抗肿瘤细胞毒活性.对这163株放线菌的16SrDNA序列分析表明,它们分属于链霉菌属(89%)、小单孢菌属(6·1%)、糖单孢菌属(0·6%)、马杜拉放线菌属(3·7%)和拟诺卡氏菌属(0·6%).其中,3株链霉菌与其亲缘关系最近的菌种的16SrDNA相似性低于97%,可能是3个新种.163 actinomycetes strains were isolated from mangrove sediments in Zhangzhou, Fujian, China. Five indicator strains （Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Candida albicans , Rhizoctonia solani ） and three tumor cells （BEL7402, A549 and HL60） were used to test the anti-microbial and anti-tumor activities of the isolated strains. 42.3% of the fermentation broth of the actinomycetes showed antimicrobial activities, while 37.4% of the fermentation broth of actinomycetes possessed anti-tumor activities. 16S rDNA sequence analysis of the actinomycetes strains showed that they belong to Streptomyces （89%）, Micromo-nospora （6. 1% ）, Sacharomonospora（O. 6% ）, Actinomadura （3.7%） and Nocardiopsis（O. 6% ）. Three isolates were suggested as novel species of Streptomyces based on their 16S rDNA sequences. The highest 16S rDNA similarity could be found in each blast search and it was lower than 97%. Fig 3, Ref 15

关 键 词：放线菌 红树林 抗菌活性 抗肿瘤细胞活性 16S RDNA 

分 类 号：Q93[生物学—微生物学]