金黄色葡萄球菌中毒休克综合征毒素1基因的检测  

Detection of Toxic Shock Syndrome Toxin 1 Gene of Staphylococcus aureus

作　　者：王敏[1] 付炅[2] 李先平[1] 

机构地区：[1]中南大学湘雅二医院检验科,长沙410011 [2]中南大学湘雅医学院医学检验系,2002级长沙410013

出　　处：《中国生物制品学杂志》2008年第4期295-297,共3页Chinese Journal of Biologicals

基　　金：湖南省自然科学基金(06JJ4051)

摘　　要：目的检测编码金黄色葡萄球菌中毒休克综合征毒素l(TSST-1)的tst基因,了解该基因的携带情况。方法用PCR法对产毒素金黄色葡萄球菌染色体上编码TSST-1的tst基因进行体外扩增,并对临床分离的84株金黄色葡萄球菌进行tst基因检测及序列分析。结果对tst基因进行了检测及测序,84株受检的金黄色葡萄球菌中,tst基因阳性株占19.05%(16/84),测序结果与GenBank公布的金黄色葡萄球菌产TSST-1基因的同源性较高。结论用PCR法检测tst基因,具有良好的重复性、特异性和敏感性。tst基因阳性株在临床分离的金黄色葡萄球菌中占有较高的比例。Objective To detect the tst gene encoding toxic shock syndrome toxin 1 （TSST-1） of Staphylococcus aureus and explore the carrier condition of the gene. Methods The tst gene encoding TSST-1 of S. aureus was amplified in vitro by PCR. Meanwhile, the tst gene in 84 S. aureus strains isolated in clinic were detected by PCR and sequenced. Results The tst gene was successfully detected by PCR and sequenced. Of the 84 clinical isolates of S. aureus, 16（ 19.05 % ） were positive for tst gene. The sequencing result showed that tst gene was highly homologous to the TSST- 1 gene reported in GenBank. Conclusion PCR was of good reproducibility, specificity and sensitivity in detection of tst gene. The tst gene positive strains were in a high proportion in the clinical isolates of S. aureus.

关 键 词：中毒休克综合征毒素1 PCR 金黄色葡萄球菌 

分 类 号：R378.11[医药卫生—病原生物学]