黑线仓鼠与大仓鼠线粒体DNA控制区全序列SNPs分析  

Polymorphism in mtDNA D-loop Region of the Cricetulus Barabensi and Cricetulus Trition Revealed by SNP Marker

作　　者：宋铭晶[1] 秦桢[2] 刘启龙[3] 吴瑾[2] 徐来祥[2] 

机构地区：[1]中国医学科学院实验动物研究所,北京市100021 [2]曲阜师范大学生命科学学院,山东省曲阜市273165 [3]济宁医学院附属医院,山东省济宁市272029

出　　处：《曲阜师范大学学报（自然科学版）》2008年第2期89-92,共4页Journal of Qufu Normal University(Natural Science)

基　　金：国家973计划项目(2007CB109104);国家自然科学基金(3047024730670335);山东省自然科学基金(Y2003D01);曲阜师范大学"十一五"计划省级重点建设项目资助

摘　　要：以华北地区大仓鼠和黑线仓鼠为研究对象,运用第三代DNA分子标记SNPs(single nucleotide polymorphisms)技术,研究了其线粒体DNA控制区(mtDNA D-loop)全序列的单核苷酸多态性.结果表明:在黑线仓鼠和大仓鼠种群中共检测到11个单倍型,22个多态位点,其中只有2个是颠换,其余都是转换.不同种群的核苷酸多态性不同,种群内的核苷酸多态性小于种群间的核苷酸多态性.线粒体DNA D-loop全序列适合作黑线仓鼠和大仓鼠的核苷酸多态性和遗传进化关系的分析.The mtDNA D-loop region in Cricetulus trition and Cricetulus barabensi are analyzed by SNP marker. 11 haplotypes and 22 SNP loci are found in populations of C. trition and C. barabensi. The poly-morphism within each population is smaller than that within individuals belong to each species, and the SNP polymorphism within all individuals is bigger than that within each species. These data indicated that the sequence of D-loop region may be used for polymorphism and evolution study in these two species.

关 键 词：黑线仓鼠 大仓鼠 线粒体DNA控制区 SNPS 

分 类 号：Q95-33[生物学—动物学]