杂交瘤细胞染色体制备及生物学意义初探  

Preparation of Chromosomes of Hybridoma Cell and Its Biological Significance

作　　者：秦璐璐[1] 耿建萍[1] 

机构地区：[1]扬州大学医学院,江苏省扬州市225009

出　　处：《医学理论与实践》2008年第4期375-376,共2页The Journal of Medical Theory and Practice

摘　　要：目的:改进杂交瘤细胞培养及染色体制备方法。方法:取小鼠脾细胞与骨髓瘤细胞SP2/0常规融合,用不同的培养基制备杂交瘤细胞,并在制备杂交瘤染色体时改变秋水仙素作用的时间。结果:12孔板中加入DMEM培养液的杂交瘤细胞株生长良好,其中6孔板加秋水仙素培养1h,染色体形态最佳,数目稳定。结论:DMEM培养液更适合此种杂交瘤细胞的培养。制作染色体时缩短秋水仙素培养时间,使得杂交瘤细胞染色体形态更接近于两种亲本细胞。Objective.. To improve the methods of cultivating hybridoma cells and preparating chromosomes. Methods.. The mice spleen cells were fused with SP2/0 cells to prepare the hybridoma cell in different culture medium. The acting time was changed when chromosome was prepared. Results.. Hybridoma cells growed well in 12 spots with DMEM culture. And of the 12 spots, 6 spots chromosomes had good shapes and stead numbers. Conclusion.DMEM culture is more suitable for cultivating. Shorting the culture time of colchicine can cause the shape of chromosomes to be more likely to their parents.

关 键 词：杂交瘤细胞 染色体 小鼠脾细胞 髓瘤细胞 制备方法 

分 类 号：R392-33[医药卫生—免疫学] R394[医药卫生—基础医学]