病毒性心肌炎心脏细胞因子mRNA的表达及生黄合剂防治机制的研究  

Study on cytokine mRNA expression of viral myocarditis and the mechanises of Sheng-Huang mixture

作　　者：郭亚春[1] 邢恩鸿[2] 宋鸿儒[1] 

机构地区：[1]承德医学院微免教研室,承德067000 [2]承德医学院附属医院中心实验室

出　　处：《陕西医学杂志》2008年第4期390-392,共3页Shaanxi Medical Journal

基　　金：河北省教育厅资助项目(NO.20052017)

摘　　要：目的：探讨病毒性心肌炎（VMC）小鼠心脏组织细胞因子白介素-2（IL-2）、白介素-4（IL-4）mRNA表达及生黄合剂免疫调节机制。方法：选用体重16～18g的雄性Balb／c小鼠200只，腹腔接种柯萨奇病毒B组3型（CVB3）建立VMC模型，随机分为正常组，生黄合剂治疗组、生脉饮组、抗病毒口服液组、病毒对照组。药物治疗组均灌胃给药，0．2ml／10g，2次／d。给药10d后，取小鼠心脏组织应用RT-PCR对IL-2、IL-4mRNA进行半定量分析。结果：应用生黄合剂治疗VMC后，可显著抑制IL-2mRNA，促进IL-4mRNA表达。结论：生黄舍剂可能通过调节细胞因子IL-2、IL-4mRNA表达来发挥其对VMC有益的免疫调节作用。Objective：the cytokines MRNA experession（IL-2,IL-4） was investigated in heart tissues with RT-PCR in all survived mice treated with Sheng-Huang nixture and to explore the possible immunological regulative mechanism of Sheng-Huang nixture to VMCo Metheds：Balb/c male mice ,weight 16～18g,Except normal control group, 0.1ml of CVB3 （ 800TCID50/0.1ml） was given to each mouse and the infective model of VMC was made. All the mice were divide into 7 groups at random,such as normal control group,Sheng-Huang reagent group, Shengmai Yin , kangbingdukoufuyie and viral control group. The drug were lavaged with the dosages of 0.2ml/10g in different groups. 10 mice in each group at the 10th day after treated, their hearts were isolated with aseptic technique and used detect the IL-2, IL-4mRNA with semi-quantitation PCR. Results： Sheng-Huang mixture singnificantly inhibit the expression of IL-2mRNA and increase the levels of IL-4mRNA. Conclusion：regulating the IL-2,IL-4mRNA levels of cytokines may be on of the mechanisms of sheng-huang mixture treating VMC.

关 键 词：心肌炎/病理生理学 细胞因子类 白细胞介素2 白细胞介素4 动物 实验小鼠 

分 类 号：R259.422.1[医药卫生—中西医结合] R285.5[医药卫生—中医内科学]