用TK基因和HGPRT基因突变试验检测人参皂甙Rg1的抗诱变性  

DETECTION OF ANTIMUTAGENICITY OF GINSENOSIDE RG1 BY USING TK GENE AND HGPRT GENE MUTATION TEST

作　　者：雷方[1] 周炯林[1] 张立实[1] 

机构地区：[1]四川大学华西公共卫生学院营养与食品卫生学教研室,成都610041

出　　处：《现代预防医学》2008年第8期1525-1527,共3页Modern Preventive Medicine

基　　金："十一五"国家科技支撑计划重大项目(2006BAK02A07;2006BAD27B08)

摘　　要：[目的]用TK基因和HGPRT基因突变试验评价人参皂甙Rg1的抗诱变性,为其进一步的开发利用提供资料。[方法]用人参皂甙Rg10.391、3.125、25、200μg/ml与阳性致突变物甲基磺酸甲酯(MMS)5μg/ml同时处理TK6细胞4h,采用微孔板法检测tk和hgprt两个位点突变频率。[结果]随受试物剂量的增加,人参皂甙Rg1拮抗MMS诱变性的作用增大,表现在tk和hgprt两个位点突变频率均较MMS组降低,差异有统计学意义(P﹤0.05)。[结论]人参皂甙Rg1具有拮抗MMS诱导的tk基因和hgprt基因突变的作用;TK6细胞可用于TK基因和HGPRT基因突变的同时检测,但TK基因突变试验比HGPRT基因突变试验更为敏感。[Objective] To evaluate the antimutagenicity of Ginsenoside Rgl by using TK gene and HGPRT gene mutation tests. [ Methods] The TK6 human lymphoid cells were exposed to ginsenoside Rgl at the concentrations of 0.391, 3.125, 25, 200μg/ml and simultaneously with 5μg/ml of methyl methanesulfonate （MMS） for 4 hours. Determination of mutant frequency of tk locus and hgprt locus were performed by using the mierowell method. [ Results] The effective of panaxoside Rg1 for inhibiting MMS mutagenicity showed increased with the increasing of dose. When compared with the MMS control, the mutant frequency of tk locus and hgprt locus after treatment with ginsenoside Rgl significandy depressed. [ Conclusion] Ginsenoside Rgl has obvious inhibiting effect on tk gene and hgprt gene mutation induced by MMS. TK6 can be used for both of TK and HGPRT gene mutation test, but TK gene mutation test showed more sensitive than HGPRT gene mutation test in detection of antimutagenicity of chemicals.

关 键 词：人参皂甙RG1 TK6细胞 TK基因突变试验 HGPRT基因突变试验 

分 类 号：R286.0[医药卫生—中药学]