实验大鼠轻中型闭合性脑损伤昏迷指标与分级标准  

Coma Criterion and classification Standard of Mild and Moderate Traumatic Brain Injury in Rats

作　　者：于建云[1] 李俊祥[1] 郭泽云[2] 赵小林[1] 赵旭东[3] 

机构地区：[1]昆明医学院法医学院 [2]昆明医学院组胚教研室,云南昆明650031 [3]昆明医学院精神病学教研室,云南昆明650031

出　　处：《法医学杂志》2008年第1期8-11,共4页Journal of Forensic Medicine

基　　金：国家自然科学基金资助项目(39160076;30160027);云南省自然科学基金资助项目(2001C0048M)

摘　　要：目的为使实验室间的研究数据可比,特建立大鼠轻型和中型闭合性脑损伤分级的昏迷量化标准。方法利用自制金属单摆装置复制大鼠脑损伤动物模型,按照大鼠脑损伤后昏迷反应程度进行呼吸暂停时间,角膜反射、外耳道刺激反应、翻正反射及针刺疼痛反应消失后恢复时间的实际测量与评分,结合肉眼颅脑解剖检查进行验证,对前述指标的实测值和评分赋值数据进行多元判别回归分析,建立判别方程。结果应用实测值和评分值进行轻型脑损伤判别的概率分别是88.9%和91.9%。结论该标准是一个较理想实用的大鼠轻、中型脑损伤分级标准。Objective To set up a classification standard of mild and moderate traumatic brain injury,for the purpose of reliable data comparison derived from different laboratories. Methods Traumatic brain injury （TBI） in rats was prepared by using a metallic pendulum-striker device. After injury, five variable parameters including the time of apnea and the areflexia, time of corneal reflex, external auditory canal stung reaction, body-righting reflex and needling reaction were determined and scored by using rat coma criterion. These data were judged and classified into mild and moderate head injury by brain patho-anatomy changes. Then the data were used to set up a multivariate discriminate equation.Results The distinguished probability of mild and moderate TBI according to actual direct measured value and the criterion were 88.9% and 91.9%, respectively. Conclusion This method is able to classify mild and moderate TBI in rats.

关 键 词：法医病理学 大鼠 脑损伤 昏迷 分级标准 

分 类 号：DF795.1[医药卫生—法医学]