邻苯二甲酸二丁酯对小鼠骨髓微核和精子形态的影响  

Effect of Dibutyl Phthalate on the Microkemel and Sperm Cell Abnormalities in Mice

作　　者：李玲[1] 刘莉[1] 刘桂株[1] 

机构地区：[1]宁夏医学院公共卫生学院,银川750004

出　　处：《宁夏医学院学报》2008年第2期194-195,共2页Journal of Ningxia Medical College

摘　　要：目的了解邻苯二甲酸二丁酯(DBP)对微核试验和精子畸形试验的影响。方法将昆明种小鼠随机分为5组,DBP染毒剂量为500mg/kg(低剂量组),1000mg/kg(中剂量组)和2000mg/kg(高剂量组),以及阴性对照组(玉米油)和阳性对照组(环磷酰胺,40mg/kg),每只小鼠计数1000个骨髓嗜多染红细胞(PCE)和每个剂量组的小鼠计数1000个精子的形态。结果中、高剂量组微核率与阴性对照组相比明显升高(P<0.05),且各剂量组微核率随染毒浓度的增加而升高,呈现剂量-反应关系(r=0.937,P<0.01);中、高剂量组精子畸形率与阴性对照组比较差异有统计学意义(P<0.05),且各剂量组精子畸形率随染毒浓度的增加而升高,呈剂量-反应关系(r=0.904,P<0.01),畸形精子中以无钩为最多,其次是香蕉形、尾折叠、双头,分别为4.5%、3.09%和1.32%,结论DBP染毒在一定剂量下对小鼠骨髓微核试验和精子畸形试验呈现阳性结果。Objective To explore effects of dibutyl Phthalate on the microkemel and sperm cell abnomalifies in mice. Methods 40 Kunming strain mice were divided into 5 groups, which were control group, masculinecomparison group, low dosage group（500mg/kg）, medium dosage group （1000mg/kg） and high dosage group（2000mg/kg）. The microkemel and sperm cell abnomalities were analyzed. Results The micronuclear rate in medium dosage group and high dosage group were significantly higher than that of control group （ P 〈 0.01 ）. The ratio of sperm malformation in middle group and high group were higher than those of controls （ P 〈 0.01）. The ratio of sperm malformation increased in dose-dependent manner with dose, a dose-response relationship was found. Conclusion The DBP contamination presents the positive results under the certain dosage to the mouse marrow micronucleus experiment and the spermatozoon abnormal experiment.

关 键 词：邻苯二甲酸二丁酯 微核 精子畸形 

分 类 号：R114[医药卫生—卫生毒理学]