人血小板的冷冻干燥保存：细胞内外保护剂的作用  

Freeze-Drying of Human Platelets: Influence of Intracellular and Extracellular Protectants

作　　者：周新丽[1] 张绍志[2] 陈光明[2] 

机构地区：[1]上海理工大学生物系统热科学研究所,上海200093 [2]浙江大学制冷与低温研究所,杭州310027

出　　处：《制冷学报》2008年第2期33-37,共5页Journal of Refrigeration

基　　金：国家自然科学基金资助项目(50076039)~~

摘　　要：通过液相内吞途径将13mmol/L海藻糖载入人血小板内,采用牛血清白蛋白和海藻糖作细胞外保护剂,研究细胞内海藻糖和细胞外保护剂在血小板冻干保存中分别所起的作用。实验结果表明冻干血小板的活性主要依靠细胞外保护剂提供的保护作用而得以保存,13mmol/L的细胞内海藻糖只起到有益的补充作用。在13mmol/L细胞内海藻糖和1%牛血清白蛋白+1%海藻糖细胞外保护剂同时存在的情况下,冻干保存40天的血小板复水后的数值恢复率约85%,显微镜观察细胞形态完整,对1U/ml凝血酶的聚集百分比为97.3%。13 mmol/L intracellular trehalose was loaded into human platelets through fluid-phase endocytosis pathway. Bovine serum albumin and trehalose were used as extracellular protectants. The effects ofintracellular trehalose and extracellular protectants on freeze-dried platelets were studied respectively. The experimental results show that 13mmol/L intracellular trehalose was beneficial to freeze-dried human platelets but only slightly enhanced the protection afforded by extracellular protectants. Platelets were loaded with 13mmol/L intracellular trehalose and freeze-dried in a formulation of 1% bovine serum albumin and 1% trehalose. 40 days later, the numerical recovery of rehydrated platelets was about 85%, the morphology of rehydrated platelets was intact and the aggregation percentage with thrombin （1U/mL） was 97.3%.

关 键 词：生物物理学 血小板 冷冻干燥 海藻糖 

分 类 号：Q65[生物学—生物物理学] TQ028.63[化学工程]