腺病毒DNA提纯及酶切图谱分析  

Purification of Adenovirus DNA and Restriction Pattern Analysis

作　　者：王玉兰[1] 郭彩玲[1] 牛美娟[1] 李绍贤[1] 

机构地区：[1]哈尔滨医科大学微生物学教研室

出　　处：《哈尔滨医科大学学报》1990年第4期236-238,共3页Journal of Harbin Medical University

摘　　要：本文建立了微量快速病毒DNA提纯方法。将3型腺病毒按常法用Vero细胞培养后,直接加细胞裂■液、Pronase K及RNase A消化,再用酚、氯仿去蛋白后,以冷无水乙醇沉淀,获得纯度为1.86含量为1.85μg/μl的DNA。经BamH Ⅰ、Hind Ⅲ、EcoR Ⅰ、Bgl Ⅱ及Msp Ⅰ酶切,琼脂糖电泳,获得满意结果。A rapid and small scale method for purifiction of adenovirus DNA was originated. Adenovirus type 3 yeas cultured in the Vero cells as described previously. The infected cells were lysed with cytolysis solution.Then the intracellular viral DNA was treated with pronase K, RNase A phenol and trichloromethane respectively and precipitated with cold ethanol.The yield of the viral DNA prepared directly from infected cells was sufficient for a few trial concerning restriction endonuclease analysis, and its optical density ratio at 260nm to 280nm was 1.85. The purified DNA was digested with Ba, mH Ⅰ Hind Ⅲ, Bgl Ⅱ, EcoR Ⅰ, MsP Ⅰ and electrophoresed on an agarose gel. The restriction patterns of former 4 REs was as discribed previously and the pattern of Msp Ⅰ was not reported yet.

关 键 词：腺病毒 DNA提取 酶切图谱 

分 类 号：R373.1[医药卫生—病原生物学]