红霉素耐药葡萄球菌中erm和msrA基因检测  

Detection of erm and msrA genes in staphylococci with resistance to erythromycin

作　　者：陈莲子[1] 熊自忠[1] 曹鸿霞[1] 

机构地区：[1]安徽医科大学第一附属医院感染病科

出　　处：《安徽医药》2008年第4期330-331,共2页Anhui Medical and Pharmaceutical Journal

摘　　要：目的检测临床分离的葡萄球菌(金黄色葡萄球菌和表皮葡萄球菌)对红霉素耐药的基因型别。方法在299株红霉素耐药葡萄球菌(金葡菌158株,表葡菌141株)中,聚合酶链反应(PCR)检测耐药基因ermA、ermB、ermC和m srA。结果158株红霉素耐药金葡菌中,ermA、ermB、ermC、m srA基因的阳性率分别为3.2%、7.0%、39.9%、7.0%;141株红霉素耐药表葡菌中,ermA、ermB、ermC、m srA基因的阳性率分别为2.8%、2.1%、55.3%、14.2%;少数菌株同时存在两种耐药基因。结论本地区葡萄球菌对红霉素的耐药基因主要是ermC。Aim To survey the prevalence of erm and msrA genes in staphylococci with resistance to erythromycin isolated. Methods The resistance gene of ermA, ermB, ermC and msrA in 158 S. aureus and 141 S. epidermidis with resistence to erythromycin were detected by PCR. Results The prevalence of ermA, ermB, ermC and msrA was 3.2%, 6. 9%, 39.9%, 6. 9% respectively in S. aureus, and 2. 1% ,2.1% ,55.3% ,14.2% in S. epidermidis,respectively. A few isolates carried two genes. Conclusion The most prevalent resist- ance gene was ermC in staphylococci isolates with resistance to erythromycin.

关 键 词：葡萄球菌 红霉素 耐药 基因型 

分 类 号：R450[医药卫生—治疗学]