慢病毒转染人骨髓间质干细胞研究  

Lentiviral transfection of mesenchymal stem cells derived from human bone marrow and its biological characteristics

作　　者：张徐[1] 钱晖[1] 朱伟[1] 曹慧玲[1] 徐学静[1] 司煜安[1] 王梅[1] 许文荣[1] 

机构地区：[1]江苏大学医学技术学院,江苏镇江212013

出　　处：《江苏大学学报（医学版）》2008年第2期127-130,134,I0002,共6页Journal of Jiangsu University：Medicine Edition

基　　金：国家自然科学基金资助项目(30471938);卫生部科研基金资助项目(WKJ2005-2-024);江苏大学创新预研基金资助项目(04CX07);江苏省医学领军人才资助项目

摘　　要：目的:分离培养人骨髓来源间质干细胞(mesenchymal stem cells,MSCs)并进行慢病毒载体介导的基因转染研究。方法:采取全骨髓贴壁培养法分离培养人骨髓MSCs。利用脂质体法进行慢病毒感染人骨髓MSCs。结果:分离培养出人骨髓来源的MSCs,并用携带绿色荧光蛋白(EGFP)报告基因的慢病毒成功感染人骨髓MSCs。结论:获得人骨髓MSCs并进行慢病毒载体介导的基因修饰,为间质干细胞基因工程和组织工程研究奠定了基础。Objective： To isolate and culture mesenchymal stem cells（MSCs） derived from human bone marrow and transfect the cells by lentiviral vectors. Methods： The tissue adherent culture method was used to isolate MSCs from human bone marrow. The MSCs were transfected by lentiviral vectors using lipofectamine 2000. Results： Human bone marrow MSCs were successfully isolated and efficiently transfected by EGFP-expressing lentiviral vectors. Conclusion： Human bone marrow MSCs have been transfected by lentivirus, which will play a role in researching on the function of MSCs in gene and tissue engineering.

关 键 词：间质干细胞 慢病毒 转染 

分 类 号：R392.2[医药卫生—免疫学] R373[医药卫生—基础医学]