扶正治标法对青少年花粉症患者相关基因表达的影响  

Influence on related gene express in hobbledehoy patients with hay fever by herb

作　　者：贯剑[1] 郑湘瑞[2] 何裕民[3] 周飞 

机构地区：[1]江苏大学附属上海第八医院中医科,上海200235 [2]河南中医学院基础医学院,河南郑州450008 [3]上海中医药大学基础医学院,上海201203 [4]上海市针灸经络研究中心,上海201203

出　　处：《江苏大学学报（医学版）》2008年第2期135-138,共4页Journal of Jiangsu University：Medicine Edition

基　　金：国家自然科学基金资助项目(30100234)

摘　　要：目的:研究中药治疗花粉症的作用机理。方法:选择50例花粉症患者,口服敏康片,治疗6个月后观察疗效及FcεRIβ,IL-4Rα,IL-5Rα,HLA-A基因表达情况,并以20例变应性皮炎和19例正常人为对照,用Northern blot检测FcεRIβ,IL-4Rα,IL-5Rα的变化。用RT-PCR法检测HLA-A,计算相对光密度值。结果:花粉症组患者症状有明显改善(P<0.05),治疗前后血清总IgE比较差异有统计学意义(P<0.01),花粉症组4个基因的表达治疗前与正常人比较,差异均具有统计学意义(P<0.01),治疗后与治疗前比较差异有统计学意义(P<0.01)。变应性皮炎组治疗前FcεRIβ,IL-4Rα与正常组比较差异有统计学意义(P<0.05),治疗前后FcεRIβ比较,差异有统计学意义(P<0.05)。鼻炎组、皮炎组HLA-A相对光密度值均较正常组高,两组治疗前后也有不同程度的改善(P<0.05)。结论:中药可以调整花粉症患者相关基因表达,改善患者过敏体质,缓解临床症状。Objective： To evaluate the efficacy of Minkang tablet on gene hay fever. Methods： The trial involved 50 patients with hay fever, 20 patients express in the patients with with atopic dermatitis, Minkang tablet（three capsule, twice a day, 6 months） was given to patients with hay fever（50）,also 19 as normal as control. Results ： The symptom and IgE after medication had difference highly statistically significant before medication. The FceRIβ,IL-4Ra, IL-5Ra, HLA-A related with atopy before and after medication, FceRIβ,IL-4Ra,IL-5Ra, HLA-A in the patients with hay fever and in normal were different highly statistically significant（ P 〈 0.01 ） before medication; and genes express improved significantly （P 〈 0.01 ） after medication. FceRIβ ,IL-4Ra of the patients with atopic dermatitis and in normal were statistically sig- nificant（ P 〈 0.05 ） before medication, FceRIβ improved significantly（ P 〈 0.05 ） after medication. Conclusion： Minkang tablet was effective in controlling gene express on patients with hay fever.

关 键 词：花粉症 敏康片 基因表达 NORTHERN BLOT 中医药疗法 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]