中国人群Kell血型K阳性的分子特征及分型试剂盒的研制和应用  

Molecular Basis of K+ in Chinese,Making and Application of PCR-SSP Kit

作　　者：王玲玲[1] 杨颖[1] 朱自严[1] 

机构地区：[1]上海市血液中心,200051

出　　处：《临床输血与检验》2008年第2期110-113,共4页Journal of Clinical Transfusion and Laboratory Medicine

基　　金：上海市卫生局科研课题基金(No054028)资助

摘　　要：目的了解中国人群Kell血型中K+型的分子生物学特征,并研制序列特异性引物-聚合酶链(PCR-SSP)试剂盒,为制订血型检测策略提供依据。方法测序分析血清学鉴定K+标本,确定K+突变类型,根据突变类型分别设计K、k基因型的特异性引物,研制PCR-SSP扩增试剂盒,同时分析100名上海地区献血者、23名外籍献血者、72名新疆地区维吾尔族献血人群的K/k基因型,并随机抽取阳、阴性标本进行测序。结果血清学鉴定为K+的突变类型为C698T突变;经研制的K/kPCR-SSP试剂盒可以特异性扩增K基因和k基因。1例新疆标本基因型为K+k+;3例外籍献血者标本基因型为K+k+,其余均为K-k+。结论本研究中所有中国人K+的分子生物学特征与西方人一致,经研制的PCR-SSP试剂盒可直观有效地检测KELK/k基因型。不同种族、不同地区献血者K+频率的差异有显著性,制订相关检测法规或条例时应有所考虑,建议外国人献血、产前检查时加做K+检测。Objective To know about the molecular basis of the K＋ in Chinese and make the PCR-SSP kit to analyze the frequency of K＋ genotype in 3 different donor groups. Methods The K＋ phenotype samples positive controls were sequenced to check the mutation type in Chinese,then 2 pairs of specific primers for K and k genes were designed for this mutation and the PCR-SSP kit was made to detect K and k genes, then this kit was used to analyze three different donor groups including 100 Shanghainese, 72 Uigurs and 23 foreigners respectively. All the K＋ samples and some K- samples were sequenced for confirming. Results The results showed that all the K＋ mutation in our study was C698T type,just the same as reported. The kit we made was useful in detecting the K gene and the k gene. Among three groups, 1 Uigur and 3 foreigners were found as K＋ with the genotype of K＋k＋ ,while the others were K-k＋. Conclusions All the Chinese K＋ in this study have the same molecular basis as the most caucasion K＋ ,and th＇： kit we made is specific and useful. Different population from different ethnicitles or regions varied with different frequency of K＋gene. For the foreigners living in China, we suggested the regulations about transfusion relating tests or prenatal tests should consider the Kell blood type.

关 键 词：序列特异性引物-聚合酶链反应 KEL基因 血型检测 K+ K+ 

分 类 号：R392.11[医药卫生—免疫学] R457.11[医药卫生—基础医学]