油酸肺损伤时肺泡内液体清除和肺泡II型细胞内cAMP、cGMP水平的变化  

Alveolar fluid clearance and changes of alveolar type Ⅱ cell cAMP and cGMP levels in rats with oleic acid-induced acute respiratory distress syndrome

作　　者：何琳[1] 李涛平[2] 朱丽华[2] 

机构地区：[1]南方医科大学南方医院急诊科,广东广州510515 [2]南方医科大学南方医院呼吸科,广东广州510515

出　　处：《南方医科大学学报》2008年第4期513-516,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(30471720);广东省自然科学基金(04020458)~~

摘　　要：目的观察油酸致大鼠急性呼吸窘迫综合症(ARDS)模型中肺泡II型细胞cAMP、cGMP水平的变化及特布他林的作用。方法随机将SD大鼠分为对照组、ARDS组、特布他林组。用放射免疫分析法测定肺泡II型上皮细胞内cAMP、cGMP水平;应用重力法测定血管外肺水含量(EVLW)。结果ARDS组大鼠肺泡II型上皮细胞内cAMP明显降低,cGMP水平升高,EVLW明显增多。给予特布他林治疗后,EVLW下降,肺泡II型细胞内cAMP含量升高,cGMP变化不明显。结论油酸致ARDS时,肺水清除能力下降。特布他林可通过提高肺泡II型细胞内cAMP水平提高肺氷清除能力。肺泡II型细胞内cAMP、cGMP水平变化参与急性肺损伤的分子生物学发病机制。Objective To observe the changes of the levels of cyclic adenosine monophosphate （cAMP） and cyclic guanosine monophosphate （cGMP） in acutely isolated alveolar type Ⅱ （ATⅡ） cells from rats with acute respiratory distress syndrome （ARDS）, and test the effects ofterbutaline on the cAMP and cGMP levels. Methods SD rats were randomized into the control, ARDS, and terbutaline treatment groups, in which the cAMP and cGMP contents in the ATII cells were measured using radioimmunoassay, and the extravascular lung water （EVLW） content was quantified with gravimetric measurement. Results The cAMP level in the ATⅡ cells was significantly lowered whereas cGMP level and EVLW content increased in rats with oleic acid-induced ARDS. In terbutaline-treated rats with ARDS, the EVLW content were lower than that of non-treated rats, but still higher than that of the control rats. Terbutaline treatment also increased the content of cAMP but produced no significant effect on cGMP content in the ATII cells of the rats with ARDS. Conclusion Alveolar fluid clearance rate is decreased in rats with oleic acid-induced ARDS, and terbutaline can improve the capacity of alveolar fluid clearance, the mechanism of which may involve elevated cAMP content in the ATⅡ cells, cAMP and cGMP in ATⅡ cells might participate in the molecular pathogenesis of ARDS.

关 键 词：急性呼吸窘迫综合症 环磷酸鸟苷 环磷酸腺苷 血管外肺水 特布他林 

分 类 号：R563.8[医药卫生—呼吸系统]