罗氏沼虾促雄腺cDNA文库的构建及部分表达序列标签的筛选  

The construction of androgenic gland cDNA library of malaysian giant prawn Macrobrachium rosenbergii and the partial screening of expressed sequence tags

作　　者：刘萍[1] 邱高峰[1] 郑亮[1] 张玉珂[1] 

机构地区：[1]上海水产大学农业部水产种质资源与养殖生态重点开放实验室,上海200090

出　　处：《上海水产大学学报》2008年第2期129-133,共5页Journal of Shanghai Fisheries University

基　　金：上海市教委重点学科资助项目(Y1101)

摘　　要：促雄腺是甲壳动物雄体特有的内分泌腺,在性别分化中起关键作用。为了获得罗氏沼虾(Macrobrachium rosenbergii)促雄腺的表达序列标签(ESTs)及可能的功能基因全序列,运用SMART技术构建罗氏沼虾促雄腺cDNA文库,用菌落PCR的方法对cDNA文库进行初步筛选,在筛选的114个阳性重组克隆中随机挑取24个克隆测序,有12条EST序列,其中4个cDNA功能基因,4个rRNA,4个新基因。该研究用SMART技术获得全长cDNA序列的比率较高,同时对分离的四个已知基因的潜在功能进行了初步推断,为进一步筛选与罗氏沼虾促雄腺分化和发育调控相关的功能基因奠定了基础。The androgenic gland is an endocrine gland that exists only in male Crustacea. It has been shown that the androgenic gland plays a critical role in sex differentiation. In order to obtain expressed sequence tags （ESTs） and possible full-length sequences of functional genes in the androgenic gland of Malaysian giant prawn Macrobrachium rosenbergii, a cDNA library of the androgenic gland was constructed by using the SMART technology. Partial screening was performed through the cDNA library by means of the colony PCR. Of 114 positive recombinants, 24 clones were randomly picked up to sequence. The results revealed a total of 12 ESTs including 4 functional genes, 4 rRNAs and 4 new genes with no homologies in the Genbank. The present research has a high ratio of full-length cDNA sequences using the technology of SMART and makes a primary conclusion for the potential functions of the four isolated known genes. Thus, it establishes the platform for further screening more functional genes related to the differentiation and development of the androgenic gland.

关 键 词：促雄腺 罗氏沼虾 表达序列标签 CDNA文库 

分 类 号：S917[农业科学—水产科学]