磷酸化JNK介导大肠杆菌诱导人巨噬细胞系U937细胞凋亡  

Phosphorylated JNK mediated apoptosis induced by E.coli in human U937 cells

作　　者：王佳贺[1] 李淑清[2] 何平[1] 张毅[1] 李乃静[1] 李岩[1] 李慧[1] 彭扬[1] 

机构地区：[1]中国医科大学附属盛京医院干诊科,辽宁沈阳110004 [2]中国医科大学附属盛京医院医务部,辽宁沈阳110004

出　　处：《中国现代医学杂志》2008年第7期880-883,共4页China Journal of Modern Medicine

摘　　要：目的研究大肠杆菌(Escherichia coli,E.coli)诱导人巨噬细胞系U937细胞凋亡的机制以及特异性c-jun氨基末端激酶(JNK)抑制剂SP600125的保护作用。方法分别用台盼蓝染色和PI标记流式细胞仪检测E.coli感染不同时间后U937细胞的死亡率及凋亡率;Western blot方法分析JNK信号转导通路的激活情况。结果E.coli可诱导U937细胞凋亡,并且具有时间依赖性,此凋亡过程可被JNK的阻断剂SP600125阻断;在此过程中,JNK在E.coli感染10min和20min时被激活并磷酸化。结论E.coli诱导的U937细胞凋亡依赖JNK信号转导通路;SP600125通过特异性地抑制JNK活性降低E.coli诱导的U937细胞凋亡率。[Objective] To investigate the mechanism of E.coli induced U937 cell apoptosis and the effect of SP600125, a specific c-jun NH2-terminal protein kinase （JNK） inhibitor. [Methods] Apoptosis rates were examined by trypan blue stain and PI stain respectively. The phosphorylated JNK signaling pathway was analyzed by Western blot. [Results] E.cols could invuce U937 apoptosis in a time-dependent manner.SP600125, a JNK blocker, blocked the apoptosis. JNK was phosphorylated at 10min and 20 min. [Conclusion ] E.coli can induce the apoptosis of U937 cells by phosphorylation of JNK. SP600125 protects U937 cells from apoptosis induced by E.coli via inhibiting the activity of JNK.

关 键 词：大肠杆菌 细胞凋亡 U937 SP600125 JNK信号通路 

分 类 号：R378.2[医药卫生—病原生物学]