高压氧对移植的外源性人神经干细胞体内神经元分化的影响  

Effect of hyperbaric oxygenation on the differentiation of implanted human neural stem cells into neurons in vivo

作　　者：白洁[1] 栾佐[2] 周丛乐[1] 屈素清[2] 姜毅[1] 汪兆艳[2] 

机构地区：[1]北京大学第一医院儿科,北京100034 [2]海军总医院儿科,北京100037

出　　处：《中国当代儿科杂志》2008年第2期195-198,共4页Chinese Journal of Contemporary Pediatrics

摘　　要：目的观察缺氧缺血性脑损伤(HIBD)新生大鼠移植人神经干细胞(hNSCs)后高压氧(HBO)对外源性hNSCs体内神经元分化的影响。方法新生7d的SD大鼠HIBD模型制作后3d经脑室移植hNSCs,并分成移植组(n=8)和移植+HBO组(n=8)。移植+HBO组于移植后1h进行HBO治疗,每日1次,10d后断头取脑,应用免疫荧光方法检测植入细胞在皮层、海马神经元分化情况。结果移植后10d,植入细胞分化形成神经元,分布以海马、皮层为主。统计学结果表明,在皮层,两组分化的神经元计数差异无显著性(P>0.05);而在海马区,移植+HBO组神经元分化存活的数量较单纯移植组多,差异有显著性(P<0.05)。结论HIBD新生大鼠移植hNSCs后HBO促进海马区外源性hNSC向神经元的分化。Objective To study the effect of hyperbaric oxygenation （HBO） on the differentiation of the implanted human neural stem cells （ hNSCs ） into neurons in neonatal rats following hypoxic-ischemic brain damage （ HIBD ）. Methods HIBD model was prepared by ligatlon of the left common carotid artery, followed by 8% hypoxia exposure in 7- day-old Sprague-Dawley rat pups. Three days later, the rats received implantation of hNSCs into the left cerebral ventricles. Then the survived rats were randomly divided into two groups： transplantation alone and transplantation ＋ HBO （ n = 8 each ）. HBO treatment was administered （ 1.8 ATA, 1 hr once daily for 10 days ） in the transplantation ＋ HBO group 1 hr after hNSCs transplantation. Brains were removed 10 days after transplantation. Frozen coronal sections were prepared for immunofluorescence analysis to detect the neural differentiation of the transplanted cells in the cerebral cortex and hippoeampus. Results Differentiated neurons of implanted cells distributed mainly in the cortex and the hippoeampus of the injured side. There was no difference in the number of neurons in the cortex between the two groups, while the number of neurons in the hippocampus significantly increased in the transplantation ＋ HBO group compared with that in the transplantation alone group （231.4＋15.1 vs 162.6 ＋5.6; P〈0.05）. Conclusions HBO treatment may promote the differentiation of implanted hNSCs into neurons in the hippoeampus of neonatal rats following HIBD.

关 键 词：神经干细胞移植 高压氧 缺氧缺血性脑损伤 大鼠 新生 

分 类 号：R459.6[医药卫生—治疗学]