重庆地区食管鳞状细胞癌组织HPV16、18的检测及其临床意义  

HPV 16 or 18 Infection in Esophageal Squamous Cell Cancer Patients of Chongqing and Its Clinical Significance

作　　者：刘铭[1] 府伟灵[2] 张晓莉[3] 曾会昌[1] 黄君富[3] 夏梅[1] 

机构地区：[1]第三军医大学西南医院胸外科,重庆400038 [2]第三军医大学西南医院基因诊断治疗中心,重庆400038 [3]第三军医大学西南医院医院基因诊断治疗中心,重庆400038

出　　处：《中华医院感染学杂志》2008年第4期485-487,共3页Chinese Journal of Nosocomiology

摘　　要：目的探讨人类乳头状瘤病毒(HPV)感染在重庆地区食管鳞癌发病中的作用及HPV检测在食管鳞癌防治中的意义。方法采用荧光定量聚合酶链反应(PCR)法分别检测112例食管鳞状细胞癌、38例非典型增生食管上皮及74例正常食管黏膜组织中HPV-16、18型的感染状况。结果食管鳞癌组织、非典型增生上皮及正常黏膜中HPV-16DNA的检出率分别为38.4%、15.9%和5.4%;鳞癌组织中HPV-16的检出率明显高于非典型增生上皮和正常食管黏膜(P<0.05),非典型增生上皮中HPV-16的检出率虽高于正常食管黏膜,但差异无统计学意义;食管鳞癌中HPV-16的阳性检出率与其分化程度未见明显相关;所有组织标本均未检出HPV-18DNA。结论HPV-16的感染可能是重庆地区食管鳞癌发生的高危因素;积极开展防治高危型HPV感染对减低该地区食管鳞癌的发病率具有重要的意义。OBJECTIVE To investigate the role of human papillomavirus（HPV）infection in the pathogenesis of esophageal squamous cell cancer（ESCC）and the significance of test of HPV in the prevention and therapy of ESCC.METHODS HPV-16,18 DNA was examined from ESCC specimens（112 cases）,dysplasia tissues（38 cases）and normal esophageal mucosa tissues（74 cases）,respectively by using fluorescence quantitative PCR.RESULTS The detection frequencies of HPV-16 DNA in ESCC,dysplasia tissues and normal mucosa tissues were 38.4%,15.9% and 5.4%,respectively.The detection frequency of HPV-16 was significantly higher in ESCC than in dysplasia tissues and normal ones.The detection frequency of HPV-16 was also higher in dysplasia tissues than in normal mucosa,while no statistical significance was found.No significant association was observed between the detection frequency of HPV-16 and the differentiation of ESCC.HPV-18 DNA was not detected in any of tissue specimens.CONCLUSIONS HPV-16 infection may be a high-risk factor for ESCC in Chongqing,and therefore the prevention and therapy of HPV infection would be of value to the reduction of incidence of ESCC in this aera.

关 键 词：食管鳞癌 人类乳头状瘤病毒 荧光定量聚合酶链反应 

分 类 号：R373[医药卫生—病原生物学]