枳壳CBF转录激活因子基因片段的克隆  

Molecular Cloning of a Gene Fragment Encoding CBF Transcriptional Factor from Poncirus triforliata

作　　者：张杰[1] 汤浩茹[1] 罗娅[1] 张勇[1] 

机构地区：[1]四川农业大学林学园艺学院,雅安625014

出　　处：《生物技术通报》2008年第2期123-126,共4页Biotechnology Bulletin

基　　金：国家自然科学基金(No.30671454);教育部"新世纪优秀人才支持计划"(NCET-04-0905);高等学校全国百篇优秀博士学位论文作者专项基金(No.200253)

摘　　要：根据不同植物CBF同源基因的保守区设计合成简并引物,采用PCR技术首次从枳壳基因组中分离出一个DNA片段并克隆到pMD18-T载体中。序列测定和分析表明,该片段长464bp,与拟南芥3个CBF基因的核酸序列及其推导的氨基酸序列分别具有79%和67%～69%的同源性,而且推导的氨基酸序列含有同源性更高的AP2 DNA结合域和CBF蛋白的两段特征序列PKK/RPAGRxKFxETRHP和DSAWR。结果表明,本研究克隆的片段为枳壳CBF基因片段。A DNA fragment was amplified from geome of Poncirus triforliota by polymerase chain reaction using a pair of primers designed from the conserved sequences of reported CBF genes, and cloned into the vectorpMD18-T. Sequence measurement and analysis showed that the nucleotide and deduced amino acid sequence of the cloned 464bp fragment were 79% and 67% -69% identical to the three Arabidopsis CBF genes respectively.Moreover, there were an AP2 DNA-binding domain of higher identity and two signature sequences of CBF proteins, PKK/R.PAGRxKFxETRHP and DSAWP, in the deduced amino acid sequence.These result indicated that a Poncirus triforliata CBF gene fragment was obtained in this study.

关 键 词：枳壳 CBF转录激活因子 CBF基因 克隆 

分 类 号：Q943.2[生物学—植物学] S666[农业科学—果树学]