攸县油茶胚状体发生及其组织学观察  

Study on Somatic Embryogenesis and Histological Observation of Camellia yuhsienensis Hu

作　　者：彭秋发[1] 戴亚娟[1] 杜跃强 吕洪飞[1] 

机构地区：[1]浙江师范大学生化学院,浙江金华321004 [2]浙江省金华市林业局,浙江金华321004

出　　处：《安徽农业科学》2008年第8期3203-3204,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]研究以攸县油茶(Camellia yuhsienensis Hu)成熟子叶为外植体的胚状体发生情况。[方法]以子叶为外植体,接种于MS+2.0mg/L2,4-D+1.0mg/LKT培养基上,诱导胚性愈伤组织。胚性愈伤组织块转接至以MS为基本培养基,添加不同浓度6-BA和NAA的激素组合的培养基上诱导产生胚状体。取不同发育阶段的组织块制作石蜡切片,进行组织学观察。[结果]成熟子叶块接种于诱导培养基MS+2.0mg/L2,4-D+1.0mg/LKT,15d左右开始出现胚性愈伤组织。胚性愈伤组织转接至培养基为MS+2.0mg/L6-BA+0.6mg/LNAA时胚状体诱导率最高,为87.78%;无激素培养基也能诱导胚性愈伤组织产生胚状体,但诱导率极低,仅为6.67%。组织学观察表明,胚性细胞由愈伤组织表面及其内部邻近的若干细胞分化而来,经过球形胚、心形胚、子叶形胚等不同发育阶段直至成熟胚状体。[结论]诱导胚性愈伤组织分化为胚状体的最佳培养基为MS+2.0mg/L6-BA+0.6mg/LNAA。[Objective] The research aimed to investigate the somatic embryogenesis from Camellia yuhsienensis Hu using cotyledons as explants. [Method] Embryogenic calluses were obtained from cotyledons which had been cultured in MS＋2.0 mg/L 2, 4-D＋1.0 mg/L KT. Then, embryogenic calluses were incubated for somatic embryogenesis in MS basic medium with different concentrations of 6 -BA and NAA. Somatic embryos at different developmental stages were sectioned and observed under light microscope. [Result] The results showed that the embryogenic calluses were initiated within 15 d. The optimal medium for somatic embryo differentiation was MS ＋ 2.0 mg/L 6-BA ＋ 0.6 mg/L NAA and the induction frequency was 87.78%. The somatic embryos were also formed in the MS medium without phytohormones, but the induction frequency was less than 6.67%. The histological observations indicated that somatic embryos originated from epidermal cells or some cells near epidermis. The mature somatic embryos were finally formed via globular embryo, heart-shaped embryo and cotyledon-shaped embryo stages. [Conclusion] The optimal medium for somatic embryos differentiation from embryogenic callues was MS ＋2.0 mg/L 6-BA ＋ 0.6 mg/L NAA.

关 键 词：攸县油茶 胚状体 组织培养 组织学观察 

分 类 号：S565.4[农业科学—作物学]