卵巢上皮癌组织微血管密度及血管形成相关分子的检测及预后价值  

Prognostic value of microvessel density and angiogenesis-related molecules in epithelial ovarian cancer

作　　者：谭先杰[1] 郎景和[1] 楼伟珍[1] 沈铿[1] 许秀英[1] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院妇产科,北京100730

出　　处：《中华肿瘤杂志》2008年第4期274-278,共5页Chinese Journal of Oncology

基　　金：国家自然科学基金资助项目（30100199）;北京科技新星计划资助项目（2003A25）

摘　　要：目的探讨卵巢上皮癌组织微血管密度（MVD）、血管内皮生长因子（VEGF）、血小板反应素1（rpsP1）和p53蛋白表达与患者预后的关系。方法采用免疫组化法检测57例原发性卵巢上皮癌组织中VEGF、rpsP1和p53蛋白的表达情况，用CD34免疫染色后计数MVD。对VEGF、rpsP1、053蛋白和MVD与患者复发及生存时间的关系进行回顾性分析。结果卵巢上皮癌组织中VEGF、rpsP1和053蛋白表达阳性率分别为70．2％（40／57）、47．4％（27／57）和61．4％（35／57），MVD为30．3±8．5，MVD、VEGF和TSP1与复发相关（P值分别为0．030、0．025和0．026）。高MVD、VEGF和053蛋白阳性患者的中位生存时间短于低MVD、VEGF和053蛋白阴性者（P值分别为0．0187，0．010和0．005），MVD、VEGF和053蛋白是影响预后的危险因素。TSP1是影响患者预后的保护因素，其阳性患者的中位生存时间长于阴性患者（P=0．042）。多因素分析表明，MVD和p53蛋白是影响卵巢上皮癌预后的独立因素（P值分别为0．018和0．009）。结论VEGF、rpsP1和053蛋白可能参与了卵巢上皮癌的血管形成，MVD和053是影响卵巢上皮癌预后的独立因素。Objective To evaluate the correlations of microvessel density （MVD）, vascular endothelial growth factor （VEGF）, thrombospodinl （TSP1） and p53 protein with prognosis in epithelial ovarian cancer. Methods Samples from 57 patients with primary epithelial ovarian cancer were examined by immunohistochemical staining using anti-VEGF, anti-TSP1, anti-p53 and anti-CD34 antibodies. The correlation of MVD, expression of VEGF, TSP1 and p53 protein with postoperative recurrence and overall survival were analyzed retrospectively. Results VEGF, TSP1 and p53 protein was positively detected in 40 （70.2%）, 27 （47.4%） and 35 （61.4%） of those patients, respectively. The mean MVD in this series was 30. 3 ± 8.5. High MVD, positive VEGF expression and negative TSP1 expression were positively correlated with postoperative recurrence. Univariate analysis showed that patients with high MVD, positive expression of VEGF and p53 had shorter median overall survival time than those with lower MVD, negative expression of VEGF and p53 （ P = 0.0187, P = 0.010 and P = 0.005, respectively）, while TSP1 expression was revealed as a protective factor for prognosis. Patients with positive expression of TSP1 had longer median overall survival time than those with negative TSP1 expression （ P = 0. 042 ）. Multivariate analysis showed that MVD and p53 expression were two independent prognostic factors in epithelial ovarian cancer （P = 0.018 and P = 0. 009, respectively）. Conclusion VEGF, TSP1 and p53 protein may play an important role in the angiogenesis of epithelial ovarian cancer. High MVD level and p53 protein expression are two independent poor prognostic factors.

关 键 词：卵巢肿瘤 微血管密度 血管内皮生长因子 血小板反应素1 P53蛋白 血管形成 预后 

分 类 号：R686[医药卫生—骨科学]