鸭IL-18基因克隆与序列分析  

Cloning and Sequence Analysis of Duck IL-18 Gene

作　　者：韩先干[1] 黄青云[1] 尹会方[1] 邓宪方[1] 柯艳坤[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642

出　　处：《安徽农业科学》2008年第9期3545-3547,共3页Journal of Anhui Agricultural Sciences

基　　金：广东省自然基金项目(3227)

摘　　要：[目的]为深入研究鸭IL-18的基因表达及其生物学活性和分子佐剂的应用奠定基础。[方法]依据GenBank中香港鸭IL-18基因的序列设计1对特异引物,以广东水鸭脾淋巴细胞总RNA为模板进行RT-PCR扩增,克隆广东水鸭IL-18基因并进行序列分析。[结果]测序结果表明,广东水鸭的IL-18基因开放阅读框为603 bp,编码201个氨基酸,分子量为23.2 kDa。序列分析表明,所获得的广东水鸭IL-18基因与GenBank中香港鸭的核苷酸同源性为98.2%,氨基酸同源性为100%。它与鸡和火鸡的核苷酸同源性分别为86.9%和87.1%,而氨基酸同源性分别为96.5%和97.0%。[结论]进化树分析结果表明,鸭IL-18基因与鸡和火鸡进化关系较近。[Objective] The research aimed to lay the foundation for deeply studying the expression of duck IL-18 gene and its biological activity and molecular adjuvant application. [ Method ] A pair of primers was designed according to the sequences of IL-18 gene in the duck of Hongkong on GenBank website. Total RNA from spleen lymphocyte in Guangdong teal was taken as template to make RT-PCR amplification. IL-18 gene in Guangdong teal was cloned and sequenced. [Result] The sequencing results showed that the open reading frame of IL-18 gene in Guangdong teal was 603 bp, coding 201 amino acids, with the molecular weight of 23.2 kDa. The sequence analysis showed the nucleetide homology of IL-18 gene between Guangdong teal and Hongkong duck on GenBank website was 98.2% and the amino acid homology was 100%. Its nucleetide homology with chicken and turkey were 86.9% and 87.1% resp., the amino acid homology were 96.5% and 97.0% resp. [ Conclusion] The results of phylogenetic analysis showed that duck IL-18 gene had closer evolutionary relationships with that of chicken and turkey.

关 键 词：鸭IL-18基因 RT-PCR 序列分析 

分 类 号：S834[农业科学—畜牧学]