皖南某野生动物园戊型肝炎跨种感染的分析  

Cross Species Infection of Hepatitis E Virus in a Wildlife Park

作　　者：沈权[1] 蒲颖艳[1] 张文[2] 牟静[2] 曹祥荣[1] 崔立[2] 杨志彪[2] 华修国[2] 胡钧 徐春茂 

机构地区：[1]南京师范大学生命科学院,江苏南京210097 [2]上海市兽医生物技术重点实验室上海交通大学农业与生物学院,上海200240 [3]皖南野生动物救护中心,安徽休宁245400

出　　处：《安徽农业科学》2008年第9期3690-3691,3716,共3页Journal of Anhui Agricultural Sciences

基　　金：上海市科委重大项目(No.063919121)

摘　　要：从皖南某野生动物园采集包括鸟类在内的22种动物粪便样品共计38份,采用RT-nPCR法检测HEV。结果表明,28.9%的粪样呈HEV RNA阳性,其中包括多种鸟类粪样。对所有11个HEV分离株的基因序列分析表明,它们均属基因4型,同源性为96%-100%。这提示,在该野生动物中心发生了HEV的跨种间感染,并且哺乳动物HEV可以在自然条件下感染鸟类。In present study, 38 fecal samples of 22 species of animals were collected from a Wildlife Park located in eastern China. All the samples were tested for the presence of HEV RNA. The results showed that 28.9% （11/38） of the animals, including 2 avian, were infected by mammalian HEV and no animals were infected by avian HEV. Phylogenedc analysis demoustrated that the isolates we discovered belonged to genotype IV HEV and they phylo- genetically clustered together. This is the first time that cross species infection of HEV was found on zoo like location and that mammalian HEV swain infected avian.

关 键 词：戊型肝炎病毒 跨种感染 基因型 

分 类 号：S855[农业科学—临床兽医学]