中国对虾遗传连锁图谱的构建  

The construction of a genetic linkage map of marine shrimp Fenneropenaeus chinensis

作　　者：李健[1] 刘萍[1] 王清印[1] 李朝霞[1] 孙昭宁[1] 何玉英[1] 

机构地区：[1]中国水产科学研究院黄海水产研究所,山东青岛266071

出　　处：《水产学报》2008年第2期161-173,共13页Journal of Fisheries of China

基　　金：国家"八六三"高技术研究发展计划(2006AA10A406);国家科技支撑计划(2006BADO1A13)

摘　　要：利用RAPD、SSR和AFLP三种标记技术结合"拟测交"策略,以中国对虾"黄海1号"雌虾与野生雄虾作为亲本进行单对杂交产生的F1家系为作图群体,初步构建了中国对虾雌、雄性遗传连锁图谱。对460个RAPD引物和44对SSR引物进行筛选,共选出61个RAPD和20对SSR引物,结合88对AFLP引物组合对父母本和82个F1个体进行了遗传分析。共得到783个分离标记(RAPD标记237个,微卫星标记45个,AFLP标记501个),761个标记用于连锁分析。雌性图谱包括40个连锁群和15个三联体,20个连锁对,标记间平均间隔为12.5cM,图谱共覆盖2835.5cM,覆盖率为73.5%;雄性图谱包括41个连锁群和6个三联体,12个连锁对,标记间平均间隔为11.9cM,图谱共覆盖2776.7cM,覆盖率为73.3%。中国对虾遗传图谱的构建为其分子标记辅助育种、比较基因组作图及数量性状位点(QTL)的定位与克隆奠定了基础。Chinese shrimp （ Fenneropenaeus chinensis） is one of the most important fanned species in China. During 1986- 1992, China was a world leader in both the artificial propagation of shrimp larvae and harvest of cultured shrimp. However, the epizootic breakout in 1993 caused a serious decrease in the total harvest of cultured shrimp. One of the measures was to genetically improve the farming species. Many molecular genetics of F. chinensis were carried out to accelerate the breeding programs such as the analysis of mitochondrial genome and population genetic structures, screening of genetic markers linked to growth traits, etc. While the construction of the linkage map has been relatively slow. In the study, the primary genetic linkage maps of F. chinensis were constructed using ＂two way pseudo testcross＂ strategy with RAPD, SSR and AFLP markers. Parents and F1 progeny were used as segregating population. 61 RAPD primers, 20 pairs of microsatellite primers screened from 460 RAPD primers and 44 pairs of SSR palmers combined 88 pairs AFLP primers were used to analyze the parents and 82 progeny of the mapping family. 783（237 RAPD, 45 microsatellites and 501 AFLPs）were segregating markers in which 761 markers were used to analysis. The female linkage map included 40 linkage groups, 15 triplets and 20 doublets, spanning 2835.5 cM with the average marker density of 12.5 cM, and the observed coverage was 73.5%. The male linkage map included 41 linkage groups, 6 triplets and 12 doublets, spanning 2776.7 cM, and the observed coverage was 73.3%. In addition, 5 microsatellite markers were mapped in both female and male linkage map that can be used for the construction of sexual linkage map of F. chinensis. Genetic linkage maps play a prominent role in many areas of genetics, including QTL analysis, map-based cloning of genes, marker-assisted selection （MAS） and comparative genomics. The main purpose of the study was to provide a theory basis for the construction of high consistency linkage map and promote the pr

关 键 词：中国对虾 遗传连锁图谱 RAPD SSR AFLP 

分 类 号：Q75[生物学—分子生物学] S917[农业科学—水产科学]