^(12)C^(6+)重离子辐照胡麻种子初步研究  

Preliminary study of Linum usitatissmum seed irradiated by ^(12)C^(6+) heavy ions

作　　者：侯岁稳[1] 吴大利[1] 张颖聪[1] 孙兰弟[1] 李文建[1] 颉红梅[1] 梁凯[2] 张彦萍[2] 程曦[2] 秦倩倩[2] 贾瑞玲[2] 

机构地区：[1]中国科学院近代物理研究所,兰州730000 [2]兰州大学生命科学学院干旱与草地生态教育部重点实验室,兰州730000

出　　处：《辐射研究与辐射工艺学报》2008年第2期78-84,共7页Journal of Radiation Research and Radiation Processing

基　　金："西部之光"联合学者项目(XL050616);教育部新世纪优秀人才支持计划;甘肃省农业生物技术专项(GNSW-2006-16)资助

摘　　要：本文研究了6×108cm-2、1.8×109cm-2和3.6×109cm-2的12C6+重离子束辐照对胡麻种子M1代生物学性状和DNA分子多态性等方面的影响。6×108cm-2辐照处理可引起胡麻发芽率提高,促进植株株高,增强花粉活力。同时辐照处理使胡麻种子千粒重和含油量有不同程度提高,辐射剂量越高,两者数值越大,3.6×109cm-2辐射剂量的胡麻种子千粒重和含油量与对照组的相比分别高出了16.5%和19.9%,此外在此剂量处发现了花粉发生了形态变化。辐照处理对胡麻DNA分子也产生了影响,筛选出的14个随机引物可以扩增出清晰、稳定、重复性好的DNA片段,有52个是多态性DNA片段,比率为52.5%。The study of heavy ion beams as a new irradiation resource on seeds of linseed （Linum usitatissmum L.） was reported. After irradiated by ^12C^6＋ heavy ions （with irradiation dose： 6×10^8 cm^-2, 1.8×10^9 cm^-2 and 3.6×10^9 cm^-2 respectively）, the biological characters, agricultural traits, pollens and polymorphic DNA of sample was studied in Linum usitatissmum L.. The results showed that 6×10^8 cm^-2 of ^12C^6＋ could promote the linseed germination rate, plant height and pollen viability. The 1000-seed weight and the oil content of seed irradiated by ^12C^6＋ heavy ions was increased with augment of irradiation dose respectively. It has been found that 3.6×109 cm^-2 is the most effective dose for increment both of seed weight and oil content with 16.5% and 19.9%, respectively. Cytological observation reveals that the highest dose （3.6×10^9 cm^-2） can also induce alteration of pollen shape of linseed. Furthermore, RAPD（Randomly amplified polymorphic DNAs） analysis shows that 99 DNA fragments are amplificated by 14 primers and the polymorphic ratio is 52.5%.

关 键 词：^12C^6+离子 胡麻种子 生物学效应 RAPD(Randomly amplified POLYMORPHIC DNAs)分析 

分 类 号：Q691.2[生物学—生物物理学] Q691.8