百合ACC氧化酶基因全长cDNA的克隆及序列分析  

Cloning and Sequencing Full-length cDNA Encoding ACC Oxidase in Lilium

作　　者：刘鲜艳[1] 刘雅莉[1] 王跃进[1] 张宗勤[1] 

机构地区：[1]农业部西北园艺植物种质资源与遗传改良重点开放实验室,陕西省农业分子生物学重点实验室,西北农林科技大学园艺学院,陕西杨陵712100

出　　处：《西北植物学报》2008年第4期651-656,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(30371013)

摘　　要：以亚洲百合‘Polyanna’（Lilium spp．）花瓣为材料，根据已报道的百合ACC氧化酶基因片段设计1对末端扩增特异引物，采用RACE方法，获得百合ACC氧化酶基因的全长eDNA（GenBank登录号为EU296623）。该eDNA全长1152bp，具有一个954bp的开放阅读框，编码318个氨基酸。Blast搜索结果显示，百合ACC氧化酶基因核苷酸序列与其它植物已报道的ACC氧化酶基因具有71％～82％的相似性，氨基酸序列有70％～87％的相似性，聚类分析表明，与单子叶植物百合科郁金香首先聚类，其次与双子叶植物聚类，最后与单子叶禾本科和兰科植物聚类。Total RNA was extracted from Lilium ＇Polyanna＇ （Asiatic hybrid） with modified SDS/phenol method. The full length ACO cDNA of Lilium was generated via DNASTAR by Splicing the two sequences obtained from 3′RACE and 5′RACE technology with primers designed based on the EST sequence （GenBank accession number： DQ062133） that we reported. Lilium ACC oxidase cDNA （GenBank accession number EU296623） was 1 152 bp in length. Within the full-length cDNA, a clear open reading frame （ORF） was 954 nucleotides,coding 318 amino acids. The results of homologous analysis in GenBank dem- onstrated that the sequence had 71% ～82 % identity on the nucleotide sequence and 70 % ～ 87% identity on the deduced amino acid sequence. The results of phylogenetic analysis showed that ACC oxidase from Lilium and from Tulipa gesneriana L. clustered together firstly,and then came those from dicotyledon, Orchidaceae and Gramineae came subsequently.

关 键 词：ACC氧化酶 eDNA克隆 百合 基因 

分 类 号：Q785[生物学—分子生物学]