拟南芥未知功能基因At3g61870编码蛋白的叶绿体定位研究  

Chloroplast Localization of the Expressed Protein Encoded by At3g61870 in Arabidopsis thaliana

作　　者：崔永兰[1] 王鹏程[1] 杨仲南[1] 张森[1] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234

出　　处：《西北植物学报》2008年第4期662-666,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：上海市教委项目(06DZ016);上海高校选拔培养优秀青年教师科研专项基金(RE566)

摘　　要：利用反向遗传学研究方法对1个预测的拟南芥叶绿体未知功能基因At3g61870编码蛋白进行了亚细胞定位研究.通过克隆At3g61870基因5′端长229 bp的DNA片段,与绿色荧光蛋白(GFP)基因构建重组表达载体pMON530-CP-TP-GFP,经农杆菌介导转化拟南芥.转基因植株的叶肉细胞经激光共聚焦显微镜观察,叶绿素自发荧光与GFP荧光共定位于叶绿体中.结果表明,未知功能基因At3g61870编码的蛋白质为叶绿体蛋白质.Verification of predicted chloroplast protein is important to understanding of chloroplast function. The protein encoded by At3g61870 was predicted to be an Arabidopsis chloroplast protein. The 5′ 229 bp fragment of this gene was cloned and fused with GFP to construct a binary vector pMON530-CP-TP- GFP for genetic transformation. On confocal laser-scanning microscopy,green fluorescent signals were localized in chloroplasts in transgenic Arabidopsis plants indicating the 229 bp DNA fragment encoding a peptide targeted to chloroplast. The results suggest that At3g61870 encodes a chloroplast protein.

关 键 词：拟南芥 叶绿体 转运肽 融合蛋白 亚细胞定位 

分 类 号：Q789[生物学—分子生物学]