黄华属根瘤菌的16S rDNA-RFLP分析  

16S rDNA-RFLP Analysis of Rhizobia Isolated from Thermopsis

作　　者：赵美玲[1] 鞠文庭[1] 郭军康[1] 冯春生[1] 韦革宏[1] 

机构地区：[1]西北农林科技大学生命科学学院；陕西省农业分子生物学重点实验室,陕西杨陵712100

出　　处：《西北植物学报》2008年第4期680-685,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(30670372);全国优秀博士学位论文作者基金(200254);教育部新世纪优秀人才支持计划(NCET-04-0953);西北农林科技大学拔尖人才支持计划

摘　　要：采用16S rDNA-RFLP及序列分析方法,对分离自黄华属的披针叶黄华、喀什黄华和光叶黄华根瘤菌进行分析研究.结果表明,分离得到的33株根瘤菌在种水平上具有丰富的遗传多样性,它们分别归属于中慢生根瘤菌属(Mesorhizobium)、中华根瘤菌属(Sinorhizobium)、根瘤菌属(Rhizobium)和土壤杆菌属(Agrobacterium).其中,以CCNWGS0011和CCNWGS0010-1为代表的5株根瘤菌构成独立的分支,可能为潜在的新种.Thirty-three rhizobial strains isolated from root nodules of Thermopsis were studied by 16S rD- NA-RFLP and sequencing analysis. The results showed that these strains belong to Mesorhizobium,Sinorhizobium, Rhizobium and Agrobacteriurn. In addition, representative strains CCNWGS0011 and CCNWGS0010-1 of five strains formed a distinct monophyletic line within the 16S rDNA sequence phylogenetic tree,which indicated that there are putative novel species. Apparently,the results revealed a high genetic diversity among these Thermopsis rhizobia.

关 键 词：黄华属 根瘤菌 RFLP 16S RDNA序列分析 遗传多样性 

分 类 号：Q939.114[生物学—微生物学]