天然脱细胞神经支架复合骨髓间充质细胞修复坐骨神经缺损  

Acellular Nerve Allografts Combined with Mesenchymal Stromal Cells to Repair Sciatic Nerve Defects

作　　者：何红云[1] 邓仪昊[2] 佟晓杰[1] 贾桦[1] 张旭[1] 

机构地区：[1]中国医科大学基础医学院解剖学教研室,沈阳110001 [2]四川大学华西基础医学与法医学院人体解剖学教研室,成都610041

出　　处：《中国医科大学学报》2008年第2期177-180,共4页Journal of China Medical University

基　　金：辽宁省教育厅高等学校科研基金资助项目(05L449)

摘　　要：目的观察脱细胞神经支架复合骨髓间充质细胞、构建组织工程化人工神经,修复大鼠坐骨神经缺损的效果。方法贴壁法体外分离、纯化、增殖骨髓间充质细胞,取第5代细胞与脱细胞异体神经复合培养,构建移植物。SD大鼠18只,随机分为实验组,支架组,对照组。术后12周,采用坐骨神经功能指数测定、腓肠肌湿质量恢复率检测及组织学等方法评价神经缺损的修复效果。结果坐骨神经功能指数及腓肠肌湿质量恢复率的检测显示实验组优于支架组(P<0.05);组织学检查,实验组修复神经S-100蛋白表达明显强于支架组,足底皮肤单位面积内触觉小体明显多于支架组,实验组缝合神经有效神经截面积、纤维数目、纤维密度、纤维直径、髓鞘厚度均优于支架组(P<0.05),其坐骨神经缺损修复效果接近对照组。结论脱细胞神经支架复合骨髓间充质细胞可有效修复坐骨神经缺损,经脱细胞神经支架溶液诱导的骨髓间充质细胞在体内具有施万细胞的功能。Objective To evaluate the effectiveness of acellular rat sciatic nerve（ARSN） cultured with bone marrow stromal cells（BMSCs） to bridge 15-mm sciatic nerve defect with tissue engineering technique in rots. Methods 18 adult SD rats were randomly divided into 3 groups：experimental group,ARSN group and control group,6 rats in each group. A 15-mm-long nerve defect was made in the right sciatic nerve and then was bridged. The thigh bone marrow was taken out from animals of experimental group under sterile condition, and mesenchymal stromal cells were purified using adherence method, cultivated and proliferated, and then the ARSN was cultured with the mesenchymal stromal cells for 24h to repair the nerve defects in experimental group and with ARSN in the ARSN group. Twelve weeks after the operation, the experimental evaluation was made, including：Sciatic nerve function index （SFI） ;Gastrocnemius wet weight recovery rate（%）;HE staining and S-100 immunohistochemistry staining,ect. Results SFI and gastrocnemius wet weight recovery rate were higher in experimental group than in ARSN group. The S-100 immunohistochemistry staining,the density,quantity and diameter of opaque nerve fibers as well as neural sheath thickness of experiment group were all better than those of ARSN group. The amount of the tactile corpuscle in average aera was significandy higher in the experimental group than in ARSN group. Conclusion Acellular rat sciatic nerves cultured with BMSCs can repair nerve defects. BMSCs induced by ARSN have the effect of schwann cells.

关 键 词：脱细胞神经支架 骨髓间充质细胞 坐骨神经 组织工程 

分 类 号：R322.85[医药卫生—人体解剖和组织胚胎学]