氢质子波谱成像对热缺血再灌注损伤大鼠移植肝细胞再生能力的评价作用  

Using 1H-MRS to evaluate the effects of warm ischemia reperfusion injury on the regeneration of hepatic cells in orthotopic transplanted livers in rats

作　　者：张亚琴[1] 单鸿[1] 孔庆聪[1] 王劲[1] 孟晓春[1] 孙强[3] 杨扬 陈规划[1] 邢海芳 

机构地区：[1]中山大学附属第三医院放射科,广州510630 [2]器管移植中心 [3]中山大学附属第一医院器官移植中心 [4]广州通用电器医疗系统集团

出　　处：《中华肝脏病杂志》2008年第4期274-278,共5页Chinese Journal of Hepatology

基　　金：基金项目：广东省自然科学基金团队项目（05200177）;国家自然科学基金面上项目（30670594）

摘　　要：目的 探讨磁共振氢质子波谱（1H-MRS）评价大鼠原位肝移植（OLT）热缺血模型肝细胞再生能力的意义，以及热缺血对于移植肝再生能力的影响。方法以实验组热缺血10min的大鼠肝移植模型和对照组无热缺血大鼠肝移植模型各30只为研究对象，移植术后分6个时间点（6h、1d、3d、7d、14d、30d）对每只大鼠行肝脏常规T1wI、T2wI成像及氢质子波谱扫描。扫描后取肝脏，测定肝细胞的细胞增殖核抗原（PCNA）表达，同时检测肝酶代谢水平。结果 术后5个时间点实验组的肝细胞PCNA阳性率、胆碱峰与水峰的峰高比值均高于对照组，实验组和对照组胆碱峰／水峰峰高比值均与相应PCNA的阳性率呈显著正相关（r实验=0．819，P〈0．01;r对照=0．543，P〈0．01）。实验组和对照组的血清ALT、AST术后明显升高，尤以术后6h～3d最为显著，实验组的ALT、AST明显高于对照组。结论 热缺血再灌注损伤对于移植后肝细胞的再生能力有明显的影响，１H-MRS的胆碱峰可以无创伤地评价移植肝的肝细胞再生能力。Objective To investigate the prognostic value of proton magnetic resonance spectroscopy （1H-MRS） in the study of warm ischemia reperfusion injury to the regeneration of hepatic cells of the livers following their orthotopic transplantation in rats. Methods A rat orthotopic liver transplantation （OLT） model with warm ischemia, the experimental group, was established and the same was done with a control group but without warm ischemia of the livers. They were studied at 6 time points （6 hours, l, 3, 7, 14, and 30 days after OLT）. All rats took axial T1 weighted and T2 weighted imaging scans and 1H MR spectroscopies. Results The positive rate of proliferating cell nuclear antigen （PCNA） and the mean peak choline/water ratio in the experimental group were significantly higher than those in the control group and the peak choline/water ratio had a positive correlation with the positive rate of PCNA. Serum ALT and AST increased significantly after OLT, especially during the 6 hour to 3day period. The levels of ALT and AST were markedly higher in the experimental group compared to the control group. Conclusion Warm ischemia reperfusion injury of OLT has a significant effect on the regeneration of hepatic cells, and the choline peak of 1H-MRS can be used to evaluate the regeneration of hepatic cells non-invasively.

关 键 词：肝移植 肝细胞 热缺血 磁共振成像 波谱 

分 类 号：R686[医药卫生—骨科学]