祛风息痛丸对小鼠实验性疼痛的镇痛作用  

Analgesic effect of Qufeng Xitong Wan on experimental pain in mice

作　　者：张轩萍[1] 牛拴成[1] 王洁[1] 杨彩红[1] 张明升[1] 

机构地区：[1]山西医科大学药理学教研室,太原030001

出　　处：《山西医科大学学报》2008年第4期346-348,共3页Journal of Shanxi Medical University

摘　　要：目的观察祛风息痛丸的镇痛作用,为其临床应用提供一定的实验依据。方法制备小鼠实验性疼痛模型,观察祛风息痛丸的镇痛作用。①扭体法:取昆明种小鼠100只,随机分为5组,每组20只,雌雄各半。对照组灌胃给予生理盐水10ml/kg;祛风息痛丸低、中、高剂量组分别灌胃祛风息痛丸0.36,1.08,3.24g/kg。阳性对照组灌胃阿司匹林0.4g/kg。每日用药1次,10d后观察各组动物腹腔注射醋酸后5-20min内的扭体反应次数。②热板法将小鼠放在(55±1)℃的热板上,以小鼠舔后足为疼痛反应指标,观察小鼠的热痛阈值。选用痛觉阈值在5-30s的雌性小鼠50只,随机分为5组,每组10只,分组与给药方法与①同。给药10d后记录各组小鼠热板法痛阈值,比较各组间的差异。结果①扭体法实验结果显示,祛风息痛丸0.36,1.08,3.24g/kg灌胃10d使腹腔注射醋酸所致的小鼠扭体反应次数较对照组明显减少,对扭体反应次数的抑制率分别为25.4%,30.0%和43.4%(P<0.05)。②热板法实验结果显示,给药前各组小鼠热痛阈值无统计学差异(P>0.05);给药10d后祛风息痛丸各剂量组小鼠热痛阈值均较给药前显著提高(P<0.05),与对照组比较,祛风息痛丸组给药后热痛阈值显著延长(P<0.05)。结论祛风息痛丸对小鼠实验性疼痛具有良好的镇痛作用。Objective To investigate the analgesic effect of Qufeng Xitong Wan（ QFXTW） and to provide an experimental basis for its clinical use. Methods Experimental pain models of mice were established to investigate the analgesic effect of QFXTW, ① Writhing test：One hundred Kunming mice were divided into 5 groups（ n = 20 in each group,10 male and 10 female）. The mice was gavaged with physiological saline 10 ml/kg in control group,with QFXTW 0.36,1.08,3.24 g/kg in QFXTW groups,and with as- pirin 0.4 g/kg in aspirin group（positive control group）. Drugs were given once a day for 10 d. After the last administration, the writhing response times of mice were recorded during 5 - 20 rain after intraperitoneal injection of acetic acid. ②Hot plate test：A hot plate with a temperature of （55 ± 1） ℃ was used to test the pain threshold of mice. The time from putting on the hot plate to licking postpedes was defined as pain threshold time of mice. Fifty female mice with pain threshold times from 5 s to 30 s were divided into 5 groups as described above. The pain threshold time was recorded after drug administration for 10 d. Results ①Compared with control group, the writhing response times of mice significantly decreased with inhibition rates of 25.4%, 30.0% and 43.4% after garaged with QFXTW 0.36,1.08 and 3.24 g/kg for 10 d（P〈 0.05）, respectively. ②Pain threshold was not significantly different among the groups before drug administration（ P 〉0.05）. After garaged with QFXTW 0.36,1.08 and 3.24 g/kg for 10 d, the pain threshold significantly prolonged compared with that of coutrol group（P 〈 0.05）. Conclusion QFXTW has an analgesic effect on experimental pain of mice.

关 键 词：祛风息痛丸 小鼠 镇痛作用 扭体反应 热板法 

分 类 号：R962[医药卫生—药理学]