前S1蛋白阳性慢性乙型肝炎患者外周血中树突状细胞功能的研究  

Study on the Immune Function of Dendritic Cells from Peripheral Blood of Patients with Positive preS1 Protein Chronic Hepatitis B

作　　者：陈月 邹红岩[2] 方爱萍 陈洪涛 

机构地区：[1]暨南大学第二临床医院深圳市人民医院检验医学部,广东深圳518020 [2]深圳市血液中心输血研究所,广东深圳518035

出　　处：《现代检验医学杂志》2008年第2期70-72,共3页Journal of Modern Laboratory Medicine

基　　金：广东省自然科学基金(07300964)

摘　　要：目的研究前S1(preS1)蛋白阳性慢性乙型肝炎(CHB)患者外周血中树突状细胞(DC)的免疫功能。方法分别从正常健康人(10例,对照组1)、preS1蛋白阴性CHB患者(10例,对照组2)和preS1蛋白阳性CHB患者(10例,实验组)外周血中分离出单核细胞(Mo),然后将Mo与GM-CSF,IL-4体外培养12d,并于培养第6d加入IFN-γ共同培养,用MTT法测定DC刺激同种异体T细胞增殖的能力,用ELISA检测DC培养上清中TNF-α和IL-12p40+p70的含量,并与对照组比较。结果preS1蛋白阳性CHB患者组DC刺激同种异体T细胞增殖的能力,培养上清中TNF-α和IL-12p40+p70的含量,均明显低于正常健康对照组(P<0.01);而与preS1蛋白阴性对照组相比则无明显差异(P>0.05)。结论CHB患者外周血DC的免疫功能低下;在CHB患者HBV持续感染时期,其外周血DC的功能状态与PreS1阳性与否无直接相关性。Objective To study the immune function of dendritic cells （DC） from peripheral blood of patients with positive preS1 protein chronic hepatitis B（CHB）. Methods Monocytes were purified from peripheral blood of health volunteers （control group 1,10 cases） ,patients with negative preS1 protein chronic hepatitis B （control group2,10 cases） and patients with positive preS1 protein chronic hepatitis B （experimental group, 10 cases） with incubation of granulocyte/macrophage colony stimulating factor （GM-CSF）,interleukin-4 （IL-4） and interferon-γ（IFN-γ） which was added on day 6. The abilities to stimulate the proliferation of allogenic T cells were evaluated by MTT assay,and the levels of TNF-a and IL-12 p40＋p70 in culture supernatant of DCs were detected by ELISA. Results The abilities to stimulate the proliferation of allo-genic T cells by DCs and the levels of TNF-a and IL-12 p40＋p70 in culture supernatant of DCs in experimental group were significantly less than those in control group 1 （P〈0.01） ,but there was no difference versus control group2 （P〉0.05）. Conclusion It is suggested that the patients with CHB have the defective immune function of DCs. In the period of HBV persistence infection,there is no direct relativity between the function of DC from peripheral blood of CHB and posi-tive preS1 protein.

关 键 词：前S1蛋白 慢性乙型肝炎 树突状细胞 细胞因子 

分 类 号：R512.62[医药卫生—内科学] R392.12[医药卫生—临床医学]