乙肝患者血清及PBMC中HBV-DNA及前C区1896点突变的检测  

Detection of HBV-DNA and HBV preC 1896 Mutation in Sera and Mononuclear Cells of Hepatitis B Patients

作　　者：雷黎[1] 王庆保[1] 卓秀珍[1] 吕晓英[1] 

机构地区：[1]安徽中医学院第一附属医院检验中心,合肥230031

出　　处：《现代检验医学杂志》2008年第2期98-100,共3页Journal of Modern Laboratory Medicine

摘　　要：目的了解乙型肝炎患者HBV前C区1896点突变的情况。方法采用聚合酶链反应联合酶联免疫吸附试验(PCR-ELISA)检测67例乙型肝炎患者外周血单个核细胞(PBMCs)和血清中HBV-DNA及前C区1896点突变情况。结果PBMCs中HBV-DNA检出率为73.1%(49/67),1896点突变检出率为34.3%(23/67);血清HBV-DNA检出率为74.6%(50/67),1896点突变检出率为32.8%(22/67);PBMCs与血清HBV-DNA检出率无显著性差异(P>0.05)。但HBeAg(+)和HBeAb(+)无论在HBV-DNA的检出率还是在1896点突变的检出率上均有显著性差异(P<0.05)。结论乙型肝炎患者血清及PBMC中HBV-DNA及前C区1896点突变的同时检测更有利于乙肝的诊治。Objective To investigate the hepatitis B virus （HBV） preC 1896 mutation of hepatitis B patients. Methods Polymerase chain reaction -ELISA assay was used to detect HBV DNA and preC 1896 mutation in PBMCs and sera from 67 patients with hepatitis B. Results The detected positive rate of HBV-DNA was 73.1% in PBMCs and 74.6% in sera. The detected positive rate of preC 1896 mutation was 34.3% in PBMCs and 32.8% in sera. There was not significantly dif- ference between HBV-DNA and preC 1896 mutation in PBMCs and in sera. But,the detected positive rate of HBV-DNA and preC 1896 mutation between HBeAg（＋）and HBeAb（＋） were significantly difference. Conclusion It is benefit to diagnose and therapy of hepatitis B patients to detect HBV-DNA and preC 1896 mutation in PBMCs and serum.

关 键 词：乙型肝炎病毒 外周血单个核细胞 聚合酶链反应 

分 类 号：R512.62[医药卫生—内科学] Q754[医药卫生—临床医学]