芍芪多苷对迟发型变态反应小鼠细胞免疫功能的影响  

Effects of Shaoqiduogan on cellular immunity in mice with delayed-type hypersensitivity

作　　者：方笋[1] 王萌[1] 赵晓娟[1] 陈根林[1] 魏伟[1] 

机构地区：[1]安徽医科大学临床药理研究所,抗炎免疫药理学安徽省重点实验室,安徽省中药研究与开发重点研究室,抗炎免疫药物安徽省工程技术研究中心,安徽合肥230032

出　　处：《中国药理学通报》2008年第4期444-448,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30572356);安徽省自然科学基金资助项目(No050430903);安徽省科技攻关计划项目(No06013133B);高等学校博士学科点专项科研基金资助项目(No20060366003);安徽高校首批科技创新团队基金;安徽省研究实验基地优秀中青年科研带头人基金资助项目

摘　　要：目的探讨在不同免疫状态下,芍芪多苷(Shaoqid-uogan,SQDG)对迟发型变态反应(DTH)小鼠细胞免疫功能的作用。方法采用2,4-二硝基氯苯(DNCB)诱导小鼠DTH模型及环磷酰胺(Cy)诱导小鼠免疫低下或亢进模型。SQDG予小鼠连续灌胃7d。MTT法检测ConA诱导的小鼠胸腺T淋巴细胞增殖,小鼠胸腺细胞增殖法检测ConA诱导的小鼠胸腺T淋巴细胞培养上清中白细胞介素2(IL-2)的活性。结果在小鼠DTH模型,SQDG(120mg·kg-1)可降低耳肿胀、胸腺指数、ConA诱导的胸腺T淋巴细胞增殖反应以及ConA诱导的胸腺T淋巴细胞培养上清中IL-2的活性。DNCB初次致敏当日腹腔注射(ip)Cy(150mg·kg-1)可以造成DTH低下模型,致敏前3 d ip Cy(250mg·kg-1)可造成DTH亢进模型;SQDG(60和120mg·kg-1)能明显上调DTH低下模型小鼠耳肿胀、ConA诱导的小鼠胸腺T淋巴细胞增殖及小鼠胸腺T淋巴细胞培养上清中IL-2的水平;且能明显下调DTH亢进模型小鼠上述指标的水平。结论SQDG对小鼠细胞免疫具有双向调节的作用。Aim To investigate the effects of Shaoqiduogan（SQDG） on cellular immunity in mice with delayed-type hypersensitivity （ DTH ）. Methods DTH model induced by 2, 4-dinitrochlorobenzene （ DNCB ）and cyclophosphamide（Cy） suppressed and potentiated DTH model were used. SQDG（30, 60 and 120 mg· kg^-1 ） were given intragastrically （ ig ） once daily for 7 consecutive days. Concanavalin A（ Con A）-induced Tcells proliferation was measured by MTT assay. The activity of IL-2 in culture supernatants of Con A-induced T cells proliferation was measured by testing its ability to support the proliferation of Con A stimulated thymocytes which was detected by MTT assay. Results In mice with DTH, SQDG（ 120 mg· kg^-1 ）reduced ear swelling, thymus index, Con A-induced T cells proliferation and the activity of IL-2 in culture supernatants of Con A-induced T cells proliferation. The suppression and potentiation of DTH in mice were induced by given an intraperitoneal （ ip ） injection of Cy at the dose of 150mg· kg^-1 on primary sensitization day and 250 mg· kg^-1three days before primary sensitization. SQDG（60 and 120 mg· kg^-1） upregulated ear swelling, Con A-induced T cells proliferation and the activity of IL-2 in culture supernatants of T cells from mice with Cy-suppressed DTH; and downregulated levels of the aforementioned indexes in mice with Cy-potentiated DTH. Conclusion SQDG possesses a dual modulatory effect on cellular immunity in mice.

关 键 词：芍芪多苷 迟发型变态反应 胸腺淋巴细胞增殖 白细胞介素-2 

分 类 号：R332[医药卫生—人体生理学] R284.1[医药卫生—基础医学]