短柄枹种群在不同群落演替过程中的遗传多样性  

Genetic Diversity of Quercus serrata var.brevipetiolata Populations at Different Succession Stages

作　　者：顾奇萍[1] 金则新[2] 李钧敏[2] 楼文燕 

机构地区：[1]西南大学三峡库区生态环境教育部重点实验室,重庆400715 [2]台州学院生态研究所,浙江临海317000 [3]义亭中学,浙江义乌322005

出　　处：《西北林学院学报》2008年第1期18-22,共5页Journal of Northwest Forestry University

基　　金：浙江省自然科学基金项目(Y505331);浙江省教育厅科研计划项目(20040287)

摘　　要：为了解群落演替与种群遗传行为之间的紧密联系,利用RAPD技术对处于不同演替阶段森林群落(针叶林、针阔混交林、常绿阔叶林)中短柄枹种群的遗传多样性进行研究。结果表明:11个随机引物共扩增出145条条带,其中多态条带为120条,总的多态位点百分率为82.76%,平均多态位点百分率为64.14%;Shannon信息指数估算的总遗传多样性为0.474 7,平均为0.364 2。N ei指数计算的总基因多样性为0.323 4,平均为0.248 4。3个种群的的遗传多样性大小顺序为针叶林>针阔混交林>常绿阔叶林。AMOVA分析结果显示,在总的遗传变异中,30.27%的变异发生在种群间,69.73%的变异发生在种群内,种群间遗传分化系数(GST)为0.231 9,基因流(Nm)为1.653 9。种群间的遗传相似度平均为0.850 1,遗传距离平均为0.162 6,针叶林与针阔混交林中的短柄枹种群遗传相似度最高。对不同群落的短柄枹种群进行UPGMA聚类,在针叶林与针阔混交林中的短柄枹种群先聚到一起,再与常绿阔叶林中的短柄枹种群相聚。短柄枹种群这种遗传结构是其本身生物学特性的反映,同时也与群落的微环境密切相关。In order to probe into the relationship between population succession and its genetic behaver, RAPD technique（random amplified polymorphic） was used to analyze the genetic diversity and genetic differentiation of Quercus serrata vat. brevipetiolata population in three forest communities with different succession stage （coniferous forest, coniferous and broad,leaved mixed forest ,evergreen broad-leaved forest）. The results showed that s Using 11 random primers, 145 repetitive loci were prodeuced in 60 indivedual of Q. serrata vat. brevipetiolata, which 125 loci were ploymorphic. The total percetage of polymorphicloci was 82.76%. The average percentage of polymorhpic loci was 64. 14%. Estimated by Shannon information index, the total genetic diversity of three populations were 0. 474 7 with an average of 0. 364 2, while that was 0. 358 7 with an average of 0. 326 5 judged from Nei index. Judged from percentage of polymorphic loci and Shannon information index and Nei index, the genetic diversity of coniferous forest community ranked first, followed by broad-leaved mixed forest community,and then evergreen broad-leaved forest. Analysis of molecular variance （AMOVA） showed that 69. 73% of genetic variance was found within pop ulations and 20. 27 % of genetic variance resided among population. The coefficient of gene differentiation （GST） was 0. 231 9 and the gene flow （Nm） was 1. 653 9. The mean of genetic identity among 3 populations of Schima superba were 0. 850 1 and the mean of genetic distance was 0. 162 6. The genetic identity of Q. serrata vat. brevipetiolata population between the coniferous forest community and the coniferous and broad-leaved mixed forest community was the highest. The UPGMA cluster analysis based on Nei＇s genetic distance showed that coniferous forest community first gathered with coniferous and broad-leaved mixed forest community, and then with evergreen broad-leaved forest community. The genetic structure of Quercus serrata vat. brevipetiolata was influenced not only

关 键 词：短柄枹 RAPD 遗传多样性 演替 

分 类 号：S718.541[农业科学—林学]