多胺代谢与大鼠心肌缺血再灌注损伤关系的实验研究  

Myocardial polyamine metabolism and the ischemia-reperfusion injury in the rat heart

作　　者：韩丽萍[1] 徐长庆[1] 姜春明[1] 李鸿珠[1] 赵雅君[1] 龚永生[2] 杜友爱[3] 郭益民[3] 

机构地区：[1]哈尔滨医科大学病理生理教研室,150086 [2]温州医学院机能实验中心 [3]温州医学院生理教研室

出　　处：《中华心血管病杂志》2008年第4期346-349,共4页Chinese Journal of Cardiology

基　　金：国家自然科学基金资助项目（30370577,30470688）;浙江省教育厅科研项目（20070353）;温州医学院校人才启动基金（QTJ07009）

摘　　要：目的观察缺血再灌注不同时期大鼠心肌多胺代谢变化规律，探讨多胺代谢与心肌缺血再灌注损伤的关系。方法采用结扎冠状动脉方法复制大鼠在体心肌缺血再灌注损伤模型，应用逆转录聚合酶链反应（RT-PCR）、Western免疫印迹（Western blot）方法分别测定正常、缺血再灌注2h、6h、12h和24h时心肌鸟氨酸脱羧酶（ODC）和精胺／精脒乙酰转移酶（SSAT）mRNA的转录和蛋白表达水平，并用高效液相色谱仪测定多胺含量变化。结果心肌缺血再灌注后ODC和SSAT mRNA的转录和蛋白表达均上调，至再灌注24h时，与假手术组比，ODCmRNA和SSAT mRNA转录分别增加了3．1倍和3．8倍（P〈0．01），ODC和SSAT的蛋白表达分别．加了3．1倍和2．9倍（P〈0．01）。精胺、精脒和多胺总代谢池含量减少，至再灌注24h时，分别比假手术组少了33．6％、35．3％和32．9％，而腐胺多了58．9％（P〈0．01）。结论心肌缺血再灌注损伤可导致多胺代谢失衡，二者密切相关。Objective To observe the polyamines metabolism changes in rat eardiomyoeytes underwent isehemia-reperfusion （I/R） injury. Methods A branch of the descending left coronary artery was occluded to induce rat myocardial I/R injury （30 rain isehemia followed by 2 h, 6 h, 12 h, and 24 h reperfusion ）, RT-PCR and Western blot were performed to detect the expression of spermidine/spermine NI- aeetyltransferase （SSAT） and omithine decarboxylase （ODC）, the concentrations of polyamines were measured with high performance liquid chromatography in hearts with or without I/R. Results The myocardial transcription and expression of SSAT and ODC were significantly upregnlated, Compared with the sham group, ODC mRNA and SSAT mRNA respectively increased 3. 1 fold and 3, 8 fold and their proteins respectively increased 3. 1 fold and 2, 9 fold at 24 h of reperfusion （ P 〈 0, 01 ） ; the concentrations of spermidine, spermine and the total polyamine pool respectively decreased by 33. 6%, 35.3% and 32.9% while putreseine concentration increased by 58. 9% at 24 h of reperfusion （ P 〈 0. 01 ）. Conclusion Our results suggest that ischemia-reperfusion in the heart may affect polyamine metabolism and the disturbance of polyamine metabolism might thus play a critical role in myocardial I/R injury in this model.

关 键 词：多胺代谢 心肌缺血再灌注损伤 生物源多胺类 心肌鸟氨酸 

分 类 号：R686[医药卫生—骨科学]