罗格列酮抑制NE和PMA诱发心肌肥大的研究  

Inhibition of rosiglitazone on NE and PMA induced growth-promoting effects in rat cardiac myocytes in vitro

作　　者：朱肖星[1] 牛小麟[1] 马晓涛[2] 魏瑾[1] 高登峰[1] 郝广华[1] 唐发宽[3] 

机构地区：[1]西安交通大学第二附属医院心内科,陕西西安710004 [2]西安卫星测控中心,陕西西安710043 [3]解放军总医院第二附属医院心内科,北京100091

出　　处：《第四军医大学学报》2008年第8期752-755,共4页Journal of the Fourth Military Medical University

摘　　要：目的:研究罗格列酮(RSG)抑制去甲肾上腺素(NE)诱导的心肌肥大与蛋白激酶C(PKC),c-fos之间的关系.方法:在培养新生大鼠心肌细胞中,采用RSG,PKC的激动剂佛波醇酯(PMA),NE和PKC的阻断剂白屈菜季氨碱(che)观察罗格列酮在NE和PMA诱导心肌肥大中对PKC活性和c-fos的影响.结果:PMA和NE均可促进心肌细胞蛋白质的合成和3H-亮氨酸的掺入,并可增强心肌细胞PKC活性,同时使PKC从胞质移位到细胞膜,RSG和che均有抑制蛋白质合成和3H-亮氨酸掺入的作用,还有抑制心肌细胞PKC活性的作用,RSG和che还可抑制NE增加c-fos蛋白的表达.结论:RSG抑制NE引起心肌肥大与PKC和c-fos有关.AIM： To investigate the inhibitory effects of rosiglitazone（RSG） on noradrenalin（ NE ）-induced cardiac myocyte hypertrophy and the mechanism involved. METHODS： NE has been shown to be a potent stimulator for neonatal rat ventricular myocyte hypertrophy in vitro. We used the activator of PPAR γ（ RSG）, the inhibitor of protein kinase C （ PKC ） chelerythrine （ the ） to influence protein content. 3 H-leucine incorporation and activity of PKC were measured on cardiac myocytes stimulated by Chronic NE or PMA（the activator of PKC）. RESULTS： After 2-d culture, RSG not only inhibited the inducing effects of NE and PMA on the elevation of protein content and 3H-leucine incorporation on cardiac myocytes, but also decreased the enhancing effects of NE and PMA on the activity of PKC in myocytes. RSG and che decreased the enhancing effects of NE on c-fos protein expression. CONCLUSION： Inhibitory effects of RSG on NE inducing growth-promoting in myocytes are associated with PKC and c-fos pathway.

关 键 词：去甲肾上腺素 罗格列酮 蛋白激酶C 佛波醇酯类 心肌/细胞学 

分 类 号：R685.3[医药卫生—骨科学]