高效液相色谱法同时测定银黄口服液中绿原酸和黄芩苷含量  

Simultaneous determination of chlorogenic acid and baicalin in Yinhuang oral liquid by HPLC

作　　者：谭生建[1] 刘刚[1] 张捷[1] 刘昌叶[1] 潘敏翔[1] 常华[1] 

机构地区：[1]中国人民解放军第306医院药学部,北京100101

出　　处：《药学实践杂志》2008年第2期138-140,共3页Journal of Pharmaceutical Practice

摘　　要：目的：建立HPLC同时测定银黄口服液中绿原酸和黄芩苷含量的方法。方法：色谱柱用十八烷基硅烷键合硅胶为固定相（Agilent ZORBAX Eclipse XDB—C184．6×150mm，5μm）。流动相：A为甲醇-水-磷酸（50：950：5），B为甲醇；梯度洗脱程序：0～30，A：95％～35％，B：5％～65％；流速：1mL／min，检测波长327nm。结果：绿原酸和黄芩苷的保留时间分别约为10．5min和23．8min，与各自相邻峰的分离度均大于1．5。以峰面积（X）对进样浓度（Y，μg／mL）线性回归，绿原酸回归方程：Y=0．03200X-0．02160，r=0．9999，线性范围5．040～45．36μg／mL，黄芩苷回归方程：Y=0．04970X-0．4880，r=0．9999，线性范围：44．88～403．9μg／mL。绿原酸、黄芩苷回收率分别为95．7％和99．0％，RSD分别为0．43％，0．33％。结论：本方法回收率测定以及与药典法含量测定结果比较，显示本法测定结果准确，且操作简便，精密度好。Objective：To develop a HPLC quantitative method for the determination of chlorgenic acid and baicalin in Yinhuang Oral liquid. Methods： An Agilent C 18 column （ ZORBAX Eclipse XDB-C18,4. 6 × 150 mm,5 μm） was used and a mixture of methanol-water- phosphorc acid（50：950：5） was used as mobile phase A , methanol as mobile phase B, gradient elution ：0 -30, A ：95% - 35% , B ：5% - 65% . The flow rate was 1.0 mL/min and monitored at 327 nm. Results： The retention time of chlorgenic acid and baicalin were 10. 5 min and 23.8 min, respectively. The regress equation for chlorgenic acid was Y = 0. 032 00X-0. 021 60,r = 0. 999 9, and the linear range was 5. 040 -45.36 μg/mL. Baicalin was Y=0. 049 70X-0. 488 0 ,r =0. 999 9 and the linear range was 44. 88 -403.9 μg/mL. The average recovery of chlorgenic acid and baicalin were （n = 6）95.7% and 99. 0% ;RSD 0. 43% and 0. 33% respectively. Conclusion： Between the determination of this method in CP 2005, this method was sensiteve ,time-saving and accurate.

关 键 词：高效液相色谱法 银黄口服液 绿原酸 黄芩苷 

分 类 号：R917[医药卫生—药物分析学]