ILK在cGVHD狼疮样小鼠肾小管上皮细胞转分化中的作用  

Role of integrin-linked kinase in renal tubular epithelial-mesenchymal transition of mice with chronic graft versus host disease(cGVHD) lupus nephritis

作　　者：黄霞[1] 王美美[1] 

机构地区：[1]东南大学附属中大医院风湿免疫科,江苏南京210009

出　　处：《右江民族医学院学报》2008年第2期174-177,共4页Journal of Youjiang Medical University for Nationalities

摘　　要：目的观察整合素连接激酶(ILK)在慢性移植物抗宿主病(cGVHD)狼疮样小鼠肾脏中的表达,探讨其在狼疮肾炎肾小管上皮细胞转分化中的作用。方法建立cGVHD狼疮样小鼠模型,将小鼠分成正常对照组(A组)、12周模型组(B组)和16周模型组(C组),每组6只。采用逆转录聚合酶链反应(RT-PCR)法、western blot、免疫组织化学法分别检测ILK、E-钙粘蛋白和α-平滑肌肌动蛋白(α-SMA)mRNA和蛋白在肾组织中的表达。结果RT-PCR和west-ern blot方法分别检测到B、C两组小鼠肾组织ILK、α-SMA mRNA和蛋白表达较A组升高(P均<0.01),E-钙粘蛋白表达降低(P均<0.01)。ILK蛋白和mRNA表达与α-SMA表达呈正相关(r分别为0.83,0.86,P均<0.01),与E-钙粘蛋白表达呈负相关(r分别为-0.84,-0.89,P均<0.01)。免疫组化结果表明,与A组相比,B、C两组小鼠肾小管及间质均可见ILK、α-SMA蛋白显著表达,而E-钙粘蛋白的表达显著减少。结论cGVHD狼疮样小鼠肾组织中ILK蛋白及mRNA表达明显上调,可能通过介导肾小管上皮-间充质细胞转分化而参与了狼疮肾炎肾间质纤维化的进程。Objective To determine the expression of integrin-linked kinase（ILK） in renal tissue of mice with chronic graft versus host disease（cGVHD） lupus nephritis and its relationship with the epithelial-mesenchymal transition.Methods cGVHD lupus nephritis mice models were conducted.All the mice were divided into three groups： group A（control group,n=6）,group B（12-week model group,n=6） and group C（16-week model group,n=6）.The mice were killed twelve weeks later（group B） or sixteen weeks later（group C）,respectively.The protein and mRNA expressions of ILK,alpha-SMA and E-Cadherin in kidney were detected by using immunohistochemistry,western blot and RT-PCR,respectively.Results The protein and mRNA expression of E-cadherin were significantly down-regulated（P〈0.01） in group B and group C compared with group A,while expressions of ILK and alpha-SMA were up-regulated,respectively（P〈0.01）.The protein and mRNA expressions of ILK were positively correlated with alpha-SMA（r=0.83,and 0.86,all P〈0.01）,but adversely associated with E-cadherin（r=-0.84,and-0.89,all P〈0.01）.Conclusion The elevating expression of ILK may play an important role in the process of lupus nephritis through mediating the epithelial-mesenchymal transition.

关 键 词：移植物抗宿主病 狼疮肾炎 粘蛋白类 肾小管 小鼠 动物实验 

分 类 号：R593.242[医药卫生—内科学] R-33[医药卫生—临床医学]