糖尿病患者血清HCMV pp65特异性IgM表达水平  

The level of human cytomegalovirus (HCMV) pp65 IgM in the serum of patients with diabetes mellitus.

作　　者：张建英 林伟良[2] 刘文虎[2] 徐冉行[3] 凌志强 

机构地区：[1]宁波市医学科学研究所,浙江宁波315020 [2]宁波市江东区明楼街道社区卫生服务中心,浙江宁波315040 [3]宁波市第六医院,浙江宁波315040 [4]Shiga University of Medical Sciences

出　　处：《现代实用医学》2008年第3期173-175,共3页Modern Practical Medicine

基　　金：浙江省医药卫生科学研究基金项目;项目编号:2005B156

摘　　要：目的建立检测人HCMV pp65特异性IgM的酶联捕获技术,来分析糖尿病患者HCMV感染状态。方法利用抗人IgM(μ链特异性)抗体包被固相载体,采用辣根过氧化物酶(HRP)标记HCMV pp65单克隆抗体或HCMV pp65抗原,建立抗体捕获HCMV pp65特异性IgM酶联免疫技术。用该法检测727例糖尿病患者及230例健康献血员血清样本,并与HCMV-pp65抗原表达水平进行了比较。结果糖尿病患者血清HCMV pp65-IgM阳性分别为81例(11.14%,酶标抗体法)和78例(10.73%,酶标抗原法),与健康组(0.87%)相比存在统计学差异(P<0.05)。酶标抗体法与酶标抗原法在抗体捕获HCMV pp65特异性IgM检测时不存在显著性差异(P>0.05),且不受RF因子的影响。HCMV pp65-IgM与HCMV pp65蛋白表达呈现良好的相关性。结论酶联捕获HCMV pp65特异性IgM法具有简便、快速、特异和敏感等特点,可诊断HCMV活动性感染。Objective TO establish HCMV pp65：IgM antibody capture enzyme-linked immunosorbent assay （Capture-ELISA） and to detect active infection of human cytomegalovirus （HCMV） in patients with diabetes mellitus. Method Anti-human IgM with μ chain specificity was coated on microplates as capturing antibody for absorbing IgM in the specimens. HCMV pp65 antigen or McAb was labeled with HRP by NaIO4 method. IgM capture ELISA was developed and used to detect HCMV infection in 727 patients with diabetes mellitus and 230 healthy controls. The results detected by IgM capture ELISA was compared with HCMV-PP65 protein. Results Positive reactivity of HCMV pp65-IgM was 81 （11.14%, HRP labeling McAb） and 78 （10.73%, HRP labeling antigen） in 727 patients with diabetes mellitus, respectively. There was significant difference between patients with diabetes mellitus and healthy controls （p〈0.05）. However, there is no significant difference between HRP labeling McAb and HRP labeling antigen in antibody capturing HCMV pp65-IgM（p〉0.05）, and its results were not affected by RF. Results showedstrong correlation between HCMV pp65-IgM and HCMV PP65 protein. Conclusions Capture-ELISA is simple, rapid, specific and sensitive method, which can be used to detect active infection of HCMV.

关 键 词：糖尿病 巨细胞病毒疫苗 酶联免疫吸附测定 免疫酶技术 

分 类 号：R587.1[医药卫生—内分泌]