ZnT3与Aβ在APP/PS1转基因小鼠老年斑内的定位及相关性  

LOCALIZATION OF ZnT3 AND Aβ AND THEIR RELATIONSHIP IN SENILE PLAQUES OF APP/PS1 TRANSGENIC MICE

作　　者：张丽红[1] 王辛[1] 郑玮[1] 于丹[1] 王思玲[2] 王占友[1] 

机构地区：[1]中国医科大学基础医学院组织胚胎学教研室 [2]沈阳药科大学药学院,110016

出　　处：《中国组织化学与细胞化学杂志》2008年第2期122-125,共4页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金资助(30670722;30770680);高等学校博士学科点专项科研基金(20060159001)

摘　　要：目的研究锌转运体-3(zinc transporter3,ZnT3)在APP/PS1转基因小鼠大脑皮层及海马内的表达,探讨ZnT3与β-淀粉样蛋白(β-amyloid,Aβ)在老年斑内的定位分布及相关性。方法应用免疫荧光和共聚焦激光扫描显微镜观察ZnT3在APP/PS1转基因小鼠大脑内的表达及其与Aβ在老年斑内的位置关系。结果ZnT3主要分布于APP/PS1转基因小鼠大脑皮层和海马的老年斑中,海马苔藓纤维也可见ZnT3的阳性反应产物;ZnT3和Aβ双标的共聚焦激光扫描显微镜观察结果证实几乎所有Aβ老年斑中均有不同程度的ZnT3表达,且主要定位在老年斑周围的变性的神经元及其突起内,围绕老年斑的Aβ核心分布。结论ZnT3与Aβ共同表达于APP/PS1转基因小鼠老年斑内,提示ZnT3可能在老年斑内的锌离子的聚集过程中起着重要的调节作用,进而参与了APP/PS1转基因小鼠大脑内Aβ老年斑的形成。Objective To investigate the expression of zinc transporter-3 （ZnT3） in the hippocampus and cortex of APP/PS1 transgenic mause brain, and to analyze the localization of ZnT3 and Aβ and their relationship in senile plaques. Methods Immunofluorescence and confocal laser scanning microscopy were used to analyze the expression of ZnT3 and Aβ and their positional relation in the senile plaques. Results ZnT3 was predominately distributed in the senile plaques of the APP/PS1 transgenic mouse brain, and it was also seen in the hippocampal mossy fibers. Confocal microscopic results revealed that ZnT3 was expressed in almost all the Aβ-positive plaques and located in the degenerating neurites in the periphery of the plaque, surrounding the Aβ-positive center. Conclusion The coexpression of ZnT3 and Aβ in the senile plaques of the APP/PS1 transgenic mouse brain suggests an important role of ZnT3 in the regulation of zinc accumulation during the pathological process of AD.

关 键 词：锌转运体-3 Β-淀粉样蛋白 老年斑 APP/PS1转基因小鼠 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]