针刺预治疗改善缺血性心律失常的机制--抑制心肌细胞内钙振荡的发生  

Effects of Electroacupuncture Pretreatment on Ischemic Arrhythmia and Intracellular Calcium Oscillation in Single Cardiocyte Isolated from Rats with Myocardial Ischemia and Reperfusion

作　　者：秦联萍[1] 高俊虹[1] 付卫星[1] 逯波[2] 崔海峰[2] 王玉敏[3] 喻晓春[2] 

机构地区：[1]中国中医科学院针灸研究所,北京100700 [2]中国中医科学院医学实验中心,北京100700 [3]辽宁医学院,锦州121001

出　　处：《针刺研究》2008年第2期75-79,共5页Acupuncture Research

基　　金：科技部973计划专项课题(No.2006CB504506);国家自然科学基金资助项目(No.30572417);北京市自然科学基金资助项目(No.7072053)

摘　　要：目的:观察电针预治疗对心肌缺血性心律失常以及单个心肌细胞内钙振荡的影响。方法:60只雄性大鼠随机分为假手术组(NC)、缺血再灌组(MIR)、缺血再灌+电针组(EA),每组10只。于造模前连续3 d给予双侧"内关"电针预治疗,电流强度5 mA,频率2/15 Hz,持续电刺激30 min。第3次电针结束后,结扎大鼠冠状动脉左前降支复制在体心肌缺血模型,用低灌流的方法制备离体全心缺血模型,观察各组大鼠心律失常评分,采用InCytPm 2 High Speed细胞内离子检测系统观察单个心肌细胞钙振荡情况。结果:MIR组于再灌注10 min内心律失常发生率较NC组明显升高(P<0.05);EA组心律失常发生率较MIR组明显降低(P<0.05)。NC组心肌细胞很少发生细胞内钙振荡,MIR组的心肌细胞可见频发的钙振荡,两组间比较差异有显著性意义(P<0.01);EA组的心肌细胞发生钙振荡的次数则明显低于MIR组(P<0.01)。结论:电针预治疗具有抗心律失常的作用,其效应可能是通过减少缺血心肌细胞钙振荡的发生来实现的。Objective To observe the inhibitory effects of acupuncture pretreatment on arrhythmia and intracellular calcium （[Ca^2＋]） oscillation in single cardiocyte in rats subjected to myocardial ischemia-reperfusion （MIR）. Methods Male SD rats were evenly randomized into normal control （NC）, MIR and electroacupuncture （EA） groups. MIR was performed by ligation （30 min） and reperfusion （10 min） of the left anterior descending coronary artery in vivo in rats or in the isolated and perfused rat heart subjected to a 40 min global ischemia followed by a 10 min reperfusion. EA pretreatment was applied to bilateral ＂Neiguan＂ （PC 6） for 30 min once a day in 3 consecutive days. Arrhythmic score was calculated according to Curtis＇s and Walker＇s method, and the count of[Ca^2＋] oscillation was measured by using InCytPm 2 intracellular ion detecting system. Results Both the arrhythmic score and the count of [Ca^2＋] oscillation in MIR group were considerably higher than those in NC group （P〈0.05）. While compared with MIR group, the arrhythmia score and the count of [Ca^2＋] oscillation in EA group were decreased significantly （P〈0.05）. Conclusion Pretreatment of EA can effectively resist MIR-induced arrhythmia and intracellular calcium oscillation in the rat.

关 键 词：心肌缺血再灌注 缺血性心律失常 电针预治疗 钙振荡 

分 类 号：R541.7[医药卫生—心血管疾病]