CaMV基因Ⅵ在拟南芥上的遗传转化及交叉保护  

The Genetic Transformation and Genetically Engineered Cross Protection of CaMV Gene Ⅵ in Arabidopsis Thaliana

作　　者：巩振辉[1,2] 何玉科[1,2] 陈启林[1,2] 薛万新[1,2] 

机构地区：[1]西北农业大学园艺系 [2]中国科学院上海植物生理研究所

出　　处：《西北农业大学学报》1997年第4期6-12,共7页Journal of Northwest Sci-Tech University of Agriculture and Forestry（Natural Science Edition)

摘　　要：通过对ＣａＭＶＣＡＢＢ－ＢＪＩ和Ｂａｒｉ－１株系基因Ⅵ的克隆和转化，在根癌农杆菌菌种ＧＶ３１０１的介导下，利用真空渗入法获得了转化ＣＡＢＢ－ＢＪＩ和Ｂａｒｉ－１基因Ⅵ的拟南芥转基因植株。分子检测表明，基因Ⅵ已整合进拟南芥基因组并有不同程度的表达。在转基因植株中，出现了类似病毒感染症状、多生长点及花粉生活力较低等个体的变异株。而多数转基因植株（占转基因植株７０．４％）为无症状的正常植株。对转化ＣａＭＶＢａｒｉ－１株系基因Ⅵ的９个无症状转基因株系接种ＣａＭＶＣＡＢＢ－ＢＪＩ病毒抗性表明。Agrobacterium tumefaciens strain GV3101 mediated transgenic plants containing CABB BJI gene Ⅵ and Bari 1 gene Ⅵ were obtained in Arabidopsis thaliana by using the method of vaccum infiltration through the cloning and transformation of gene Ⅵ of cauliflower mosaic virus (CaMV) strains CABB BJI and Bari 1.The molecular assay showed that the gene Ⅵ was integrated into plant genomes of Arabidopsis thaliana and had expressions of various degrees.The mutants of different types,including virus symptom like phenotype,multiple growing points and lower viability of pollen,were found among the transgenic plants.Most of the transgenic plants (70.4 percent),however,were symptomless.The strain CABB BJI of CaMV artificially inoculated nine lines of symptomless transgenosis from the transformed gene Ⅵ of CaMV strain Bari 1.The results of the resistance indicated that the nine lines had various resistance reactions.

关 键 词：CaMV基因Ⅵ 遗传转化 转基因植株 表型 拟南芥 

分 类 号：Q785[生物学—分子生物学]