亚慢性乐果染毒对大鼠脑组织及血清中单胺类神经递质的影响  

Effects of 90-days Oral Dimethoate Exposure on Monoamine Neurotransmitters in Rat Brain and Serum

作　　者：姚新民[1] 邬春华[1] 吴强恩[1] 姜楠[1] 常秀丽[1] 班婷婷[1] 邵春风[1] 周志俊[1] 

机构地区：[1]复旦大学公共卫生学院劳动卫生教研室,公共卫生安全国家教育部重点实验室,上海200032

出　　处：《环境与职业医学》2008年第2期140-143,共4页Journal of Environmental and Occupational Medicine

基　　金：国家自然科学基金项目(编号：30571554);上海市曙光学者计划基金资助课题(编号：03SG04)

摘　　要：[目的]探讨有机磷农药乐果90d染毒对大鼠脑组织及血清中单胺类神经递质的影响。[方法]48只健康雄性SD大鼠随机分成4组,分别使用生理盐水,5、10、20mg/kg的乐果灌胃给药,每周染毒5d,每天1次,实验周期90d。染毒结束后断头处死大鼠,分离血清及脑组织皮层、海马,高效液相色谱(high performance liquid chromatography,HPLC)联用电化学检测器(electrochemical detector,ECD)测定大鼠脑组织及血清中单胺类神经递质去甲肾上腺素(noradrenalinebitartrate,NE)、肾上腺素(epinephrine,E)、多巴胺(dopamine,DA)、5-羟色胺(5-hydroxytryptamine,5-HT)含量。[结果]低、中、高剂量组大脑皮层的NE含量明显低于对照组,分别约为对照组水平的48.7%(P<0.01)、56.8%(P<0.01)和76.5%(P<0.05),与对照组相比差异均有统计学意义,大脑皮层的5-HT含量也明显低于对照组,分别约为对照组水平的65.4%、32.8%(P<0.01)、40.1%(P<0.05),中、高剂量组与对照组的差异有统计学意义。除中剂量组大脑皮层E含量明显低于对照组(P<0.05)外,低、中、高剂量组和对照组大脑皮层E和DA水平无明显差异。大鼠海马各单胺类递质水平有随染毒剂量的升高而降低的趋势,呈现一定的剂量-效应关系;低、中、高剂量组的NE水平分别约为对照组水平的96.9%、85.6%、76.9%(P<0.05);E水平分别约为对照组水平的87.3%、85.5%、76.2%(P<0.05);DA水平分别约为对照组水平的81.4%、69.1%、42.0%(P<0.05);5-HT水平约为对照组水平的81.4%(P<0.01)、33.9% (P<0.01)、32.9%(P<0.01),且与对照组相比差异均有统计学意义(P<0.01)。乐果染毒组与对照组血清NE和DA水平无明显差异(P>0.05);低、中、高剂量组血清中E和5-HT含量明显低于对照组,分别约为对照组水平的47.8%、59.1%、60.9%和58.9%、26.6%、23.9%,与对照组相比差异均有统计学意义(P<0.01)。[结论]乐果亚慢性染毒影响大鼠脑组织皮层、海马及其血清中单胺类递质的含量。[ Objective ]. To investigate the effects of subchronic dimethoate exposure on monoamine neurotransmitters in rat brain tissue and serum. [ Methods ] Dimethoate at the doses of 0（ saline vehicle ）, 5, 10 and 20mg/kg were administered by garage to rats 5 times a week for 90 days. At the end of the study, animals were decapitated, serum was separated and brain regions including cortex and hippocampus were quickly collected. The monoamine neurotransmitters levels such as noradrenalinebitartrate （ NE ）, 5-hydroxytryptamine （ 5-HT ）, epinephrine （ E ） and dopamine （ DA ） in brain and serum were determined by high performance liquid chromatography（ HPLC ）with electrochemical detector（ ECD ）. [ Results ] From low dose group to high dose group, contents of NE in the whole cerebral cortex decreased significantly as compared with control group（P 〈 0.01, P 〈 0.01, P 〈 0.05, respectlively ）; levels of 5-HT in the whole cerebral cortex also decreased significantly at the doses of 10, 20 mg/kg bw compared with that of the control group （ P 〈 0.01, P 〈 0.05, respectively ） .There were no significant differences in E and DA contents in the whole cerebral cortex between exposure groups and the control except a statistical significant decrease of E was observed in the medium group（ P 〈 0.05 ）. With the increase of exposure, monoamine neurotransmitters levels were decreased in hippocampus, there is a dose-effect relationship. The contents of NE, E, DA were shown to be significantly lower in the high group as compared with that of control （ P 〈 0.05 ）, and 5-HT in all exposure groups were also significantly lower in comparison with control group （P 〈 0.01 ）. There were no significant differences in NE and DA contents in the serum between exposure groups and control group, but the E and 5-HT levels in the exposure groups were significantly lower when compared with the control group （P 〈 0.01 ）. [ Conclusion ] Subchronic exposure of dimethoate could influence the re

关 键 词：乐果 大鼠 高效液相色谱 电化学检测器 单胺类神经递质 

分 类 号：R114[医药卫生—卫生毒理学]