母鼠低剂量敌百虫染毒对着床前期胚胎细胞发育及微核的影响  

Effects on Development and Micronuclei of Pre-implantation Mouse Embryos after Maternal Trichlorfon Administration at Low Levels

作　　者：周淑芳[1] 沈莉[1] 高宇[1] 施蓉[1] 周义军[1] 丁瑜[1] 王沛[1] 田英[1] 

机构地区：[1]上海交通大学公共卫生学院环境卫生教研室,上海200025

出　　处：《环境与职业医学》2008年第2期144-147,共4页Journal of Environmental and Occupational Medicine

基　　金：国家自然科学基金(编号:30571542);上海市浦江人才计划资助项目(编号:05PJ14052);上海市教委基金项目(编号:07ZZ31)

摘　　要：[目的]研究母鼠低剂量敌百虫30d染毒,对着床前期胚胎细胞发育和微核的影响。[方法]将ICR雌性小鼠随机分为敌百虫低、中、高3个染毒组(剂量分别为2、10、50mg/kg)和对照组。通过饮水方式将各染毒组小鼠染毒30d后,雌雄交配,于次晨检查阴栓,阳性当日即是为受孕第0天。对孕鼠再持续染毒至孕第3天,处死,观察胚胎发育状况、胚胎平均细胞数、碎片核、固缩核频率及微核发生率,以此评价母体敌百虫较长时间暴露对着床前期胚胎发育状况及细胞微核的影响。[结果]①各染毒组母鼠体重增长、黄体数、胚胎着床率、异常胚胎百分率与对照组比较,差别无统计学意义(P>0.05)。②染毒组着床前期小鼠胚胎平均微核数和调整后的每1 000个细胞所含微核数与对照组相比,差异无统计学意义。③高染毒组胚胎平均细胞数目、胚泡胚百分率明显减少、桑葚胚百分率明显增多(P<0.01),而低、中染毒组与对照组相比差别无统计学意义;各染毒组平均碎片核数较对照组差别无统计学意义;高染毒组和低染毒组固缩核平均数较对照组增多(P<0.01),但中染毒组与对照组比较差别未见统计学意义。[结论]在本实验剂量下,各染毒组母鼠体重增长及胚胎细胞微核未见明显影响;但高染毒组暴露可导致胚胎发育延缓,而中、低剂量组未见明显影响。[ Objective ] To assess effects on development and micronuclei ot pre-implantation mouse embryos alter matemal trichlorfon administration at low doses for 30 days. [ Methods ] Female mice were administered with trichlorfon（ 0, 2, 10, 50mg/kg ） in drinking water for 30 days, then the females were paired with a male（ two to one ）overnight and were examined for the presence of a vaginal plug in the following morning. The day on which a vaginal plug was observed was considered as Day 0 of gestation （ dg 0 ）. The pregnant mice were administered with trichlorfon continuously until they were killed on dg 3. Then, embryos were collected and evaluated for gross morphology, micronuclei （ MN ）frequency, cell number, fragmented nuclei and pycnotic nuclei. [ Results ]1. Trichlorfon exposure did not affect maternal body weight gain, corpora lutea, implantation sites, or percentage of abnormal embryos （ P 〉 0.05 ）.2. There were no significant difference in mean MN （ micronucleus ）number and MN frequency of pre-implantation mouse embryos between trichlorfon-treated groups and the control group. 3. In the 50 mg/kg trichlorfon treated group, maternal exposure decreased mean cell number of pre-implantation embryos and the blastocyst proportion, but increased the morula proportion（ P 〈 0.01 ）. There was no significant difference in mean fragmented nuclei number in all treated groups. However, mean pycnotic nuclei number were significantly increased in 50 mg/kg and 2 mg/kg trichlorfon-treated groups （ P 〈 0.01 ）. [ Conclusion ] Trichlorfon exposure did not affect maternal body weight gain nor micronuclei （ MN ） number and frequency in pre-implantation embryos. A simultaneous decrease in the cell number, the blastocyst proportion, and increase in the morula proportion may reflect an embryonic developmental disadvantage resulting from maternal treatment with triehlorfon.

关 键 词：敌百虫 着床前期胚胎 平均细胞数 微核 碎片核 固缩核 小鼠 

分 类 号：R114[医药卫生—卫生毒理学]