激光对草原龙胆自交结实及自交后代幼苗生物量的影响  

The Effect of Laser on Selfbred Seedy Quality and Selfbred Seedlings' Biomass of Eustoma grandiflorum

作　　者：张旸[1] 聂玉哲[1] 解莉楠[1] 刘福全[2] 李玉花[1] 

机构地区：[1]东北林业大学花卉生物工程研究所,黑龙江哈尔滨150040 [2]沈阳农业大学园艺系,辽宁沈阳110161

出　　处：《激光生物学报》2008年第2期191-196,共6页Acta Laser Biology Sinica

基　　金：国家先进技术引进"948"项目(2000204209);国家转基因植物研究与产业化专项课题(JY03213228);科技部"863"项目(2001AA2461)

摘　　要：研究利用激光提高草原龙胆自交结实的方法,初步探讨了激光对花粉的生物效应。对草原龙胆的花粉进行激光照射,结果表明:适宜剂量的激光照射草原龙胆花粉,能够使其萌发率显著提高,并且使自花授粉的单朵花的结籽量也显著提高;自交后代植株的生长势有较大改善,幼苗健壮,植株的生物量也显著提高。从花粉的表面结构的电镜显微观察分析,激光照射后的花粉,其花粉壁的雕纹受到伤害,网脊线有些断裂,网眼变大,花粉管原始体变长。The pollens of Eustoma grandiflorum were treated by laser for overcoming their self incompatibility and improve seedy quality. The results are as follows ： At an appropriate dosage of laser, the germination rate of pollen in Eustoma grandiflorum was increased, the numbers of seeds were increased, and the growth vigor of seedlings from self-fertilized seeds was improved remarkably to produce more biomass in the field. The surface structure of the pollen treated by laser was observed by an electron microscope. The sculpturing pattern of the treated pollen was damaged, so that some reticulate structures were disordered and the space encircled by reticulate ridges enlarged. Pollen tube primordium showed longer than that of the untreated control.

关 键 词：草原龙胆 激光 花粉 自交 

分 类 号：Q631[生物学—生物物理学]